Increased advanced oxidation of protein products and enhanced total antioxidant capacity in plasma by action of toxins of Escherichia coli STEC

被引：17

作者：

Aiassa, V. ^{[1
]}

Baronetti, J. L. ^{[1
]}

Paez, P. L. ^{[1
]}

Barnes, A. I. ^{[1
]}

Albrecht, C. ^{[2
]}

Pellarin, G. ^{[2
]}

Eraso, A. J. ^{[2
]}

Albesa, I. ^{[1
]}

机构：

[1] Univ Nacl Cordoba, Dept Farm, Fac Ciencias Quim, RA-5000 Cordoba, Argentina

[2] Univ Nacl Cordoba, Escuela Nutr, Fac Ciencias Med, RA-5000 Cordoba, Argentina

来源：

TOXICOLOGY IN VITRO | 2011年 / 25卷 / 01期

关键词：

Hemolytic Uremic Syndrome; Shiga toxin; Hemolysin; Oxidative stress; Protein oxidation; Antioxidants; STRESS; PURIFICATION; NEUTROPHILS; DAMAGE; CELLS;

D O I：

10.1016/j.tiv.2010.11.006

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 [卫生毒理学];

摘要：

Shiga toxin (Stx) and hemolysin (Hly) of Escherichia coli O157:H7 produced an increase of reactive oxygen species (ROS) in normal human blood. In vitro assays showed that stimuli of ROS with these toxins oxidized proteins to carbonyls in plasma and raised the degradation of oxidized macromolecules, with the AOPP/carbonyl relationship also increasing. The oxidative stress generated by toxins during the Hemolytic Uremic Syndrome (HUS) produced oxidation of blood proteins with a rise in advanced oxidation protein products (AOPP) in children with HUS. There was a response from the antioxidant system in these patients, evaluated through the determination of the total antioxidant capacity of plasma by the Ferric Reducing Antioxidant Power (FRAP), which reduced the stimuli of ROS during in vitro incubation with Stx or Hly. The application of natural antioxidants was sufficient to reduce in vitro the oxidative stress provoked by both toxins in blood. Published by Elsevier Ltd.

引用

页码：426 / 431

页数：6

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