Determination of α-amino nitrogen in pea protein hydrolysates:: a comparison of three analytical methods

被引:49
作者
Panasiuk, R
Amarowicz, R
Kostyra, H
Sijtsma, L
机构
[1] Polish Acad Sci, Div Food Sci, Inst Anim Reprod & Food Res, PL-10718 Olsztyn, Poland
[2] DLO, ATO, Agrotechnol Res Inst, NL-6708 PD Wageningen, Netherlands
关键词
D O I
10.1016/S0308-8146(97)00164-7
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Three spectrophotometric methods, using 2,4,6-trinitrobenzenesulphonic acid (TNBS), o-phthaldialdehyde (OPA) or ninhydrin, for the determination of alpha-amino nitrogen in pea protein isolates and hydrolysates were compared. The determined amounts of alpha-amino nitrogen differed greatly, depending on the method used. The TNBS and OPA methods produced comparable results, whereas the data obtained with the ninhydrin method were only half of the TNBS or OPA values. Colour stability, recovery of the standard from the protein matrix and reproducibility of the results were determined. The methods showed good accuracy (SE 1-3%) with recovery values of the standard (L-leucine) from the protein matrix of 91, 111 and 75% for the OPA, ninhydrin and TNBS method, respectively. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:363 / 367
页数:5
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