Trans-specific S-RNase and SFB alleles in Prunus self-incompatibility haplotypes

被引:50
作者
Sutherland, Bruce G. [1 ,2 ]
Tobutt, Kenneth R. [1 ]
Robbins, Timothy P. [2 ]
机构
[1] E Mailing Res, Maidstone ME19 6BJ, Kent, England
[2] Univ Nottingham, Sch Biosci, Plant Sci Div, Loughborough LE12 5RD, Leics, England
关键词
Prunus; self-incompatibility; SFB; S-RNase; trans-specific evolution;
D O I
10.1007/s00438-007-0300-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Self-incompatibility in the genus Prunus is controlled by two genes at the S-locus, S-RNase and SFB. Both genes exhibit the high polymorphism and high sequence diversity characteristic of plant self-incompatibility systems. Deduced polypeptide sequences of three myrobalan and three domestic plum S-RNases showed over 97% identity with S-RNases from other Prunus species, including almond, sweet cherry, Japanese apricot and Japanese plum. The second intron, which is generally highly polymorphic between alleles was also remarkably well conserved within these S-allele pairs. Degenerate consensus primers were developed and used to amplify and sequence the co-adapted polymorphic SFB alleles. Sequence comparisons also indicated high degrees of polypeptide sequence identity between three myrobalan and the three domestic plum SFB alleles and the corresponding Prunus SFB alleles. We discuss these trans-specific allele identities in terms of S-allele function, evolution of new allele specificities and Prunus taxonomy and speciation.
引用
收藏
页码:95 / 106
页数:12
相关论文
共 54 条
[1]  
[Anonymous], 1940, MANUAL CULTIVATED TR, DOI DOI 10.1038/148007A0
[2]   Determination of S-haplotypes of Japanese plum (Prunus salicina Lindl.) cultivars by PCR and cross-pollination tests [J].
Beppu, K ;
Takemoto, Y ;
Yamane, H ;
Yaegaki, H ;
Yamaguchi, M ;
Kataoka, I ;
Tao, R .
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY, 2003, 78 (03) :315-318
[3]  
Bortiri E, 2001, SYST BOT, V26, P797
[4]   Correlation of stylar ribonuclease zymograms with incompatibility alleles in sweet cherry [J].
Boskovic, R ;
Tobutt, KR .
EUPHYTICA, 1996, 90 (02) :245-250
[5]   Structural and molecular analysis of self-incompatibility in almond (Prunus dulcis) [J].
Certal, AC ;
Almeida, RB ;
Boskovic, R ;
Oliveira, MM ;
Feijó, JA .
SEXUAL PLANT REPRODUCTION, 2002, 15 (01) :13-20
[6]  
Clapham A.R., 1987, FLORA BRIT ISLES, V3rd
[7]  
CRANE M. B., 1929, JOUR POMOL & HORT SCI, V7, P276
[8]  
Dayhoff M., 1979, ATLAS PROTEIN SEQUEN, V5
[9]   Determining self-incompatibility genotypes in Belgian wild cherries [J].
De Cuyper, B ;
Sonneveld, T ;
Tobutt, KR .
MOLECULAR ECOLOGY, 2005, 14 (04) :945-955
[10]  
DESPRES C, 1994, SEX PLANT REPROD, V7, P169, DOI 10.1007/BF00228490