Characterization of the Turkey MHC chromosome through genetic and physical mapping

被引：29

作者：

Chaves, L. D. ^{[1
]}

Krueth, S. B. ^{[1
]}

Reed, K. M. ^{[1
]}

机构：

[1] Univ Minnesota, Dept Vet Biomed Sci, Coll Vet Med, St Paul, MN 55108 USA

来源：

CYTOGENETIC AND GENOME RESEARCH | 2007年 / 117卷 / 1-4期

关键词：

D O I：

10.1159/000103182

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Previous studies in the chicken have identified a single microchromosome (GGA16) containing the ribosomal DNA ( rDNA) and two genetically unlinked MHC regions, MHC-B and MHC-Y. Chicken DNA sequence from these loci was used to develop PCR primers for amplification of homologous fragments from the turkey (Meleagris gallopavo). PCR products were sequenced and overgo probes were designed to screen the CHORI 260 turkey BAC library. BAC clones corresponding to the turkey rDNA, MHC-B and MHC-Y were identified. BAC end and subclone sequencing confirmed identity and homology of the turkey BAC clones to the respective chicken loci. Based on subclone sequences, single-nucleotide polymorphisms ( SNPs) segregating within the UMN/NTBF mapping population were identified and genotyped. Analysis of SNP genotypes found the B and Y to be genetically unlinked in the turkey. Silver staining of metaphase chromosomes identified a single pair of microchromosomes with nucleolar organizer regions (NORs). Physical locations of the rDNA and MHC loci were determined by fluorescence in situ hybridization ( FISH) of the BAC clones to metaphase chromosomes. FISH clearly positioned the rDNA distal to the Y locus on the q-arm of the MHC chromosome and the MHC-B on the p-arm. An internal telomere array on the MHC chromosome separates the B and Y loci. Copyright (c) 2007 S. Karger AG, Basel

引用

页码：213 / 220

页数：8

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