Formation of PAH-DNA adducts after in vivo and vitro exposure of rats and lung cells to different commercial carbon blacks

被引:61
作者
Borm, PJA
Cakmak, G
Jermann, E
Weishaupt, C
Kempers, P
van Schooten, FJ
Oberdörster, G
Schins, RPF
机构
[1] Univ Dusseldorf, Inst Umweltmed Forsch, D-4000 Dusseldorf, Germany
[2] Gazi Univ, Dept Toxicol, Ankara, Turkey
[3] Univ Dusseldorf, Med Inst Umwelthyg, Dept Analyt Chem, D-4000 Dusseldorf, Germany
[4] Univ Maastricht, Dept Hlth Risk Anal & Toxicol, Maastricht, Netherlands
[5] Univ Rochester, Dept Environm Med, Rochester, NY 14627 USA
关键词
carbon black; bioavailability; PAHs; DNA adducts; lung;
D O I
10.1016/j.taap.2004.10.020
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Objective: The current study was designed to test the possible release and bioavailability of polycyclic aromatic hydrocarbons (PAHs) from a set of commercial carbon blacks (CBs) as well as the ability of these PAHs to form bulky DNA adducts. Methods: In four commercial CBs (Printex 90, Sterling V, N330, Lampblack 101), leaching of PAH was examined through (1) release of parent PAHs in saline with or without surfactant, and (2) PAH adducts in lung epithelial cells (A549) or in rat lungs after exposure to two CBs (Printex 90, Sterling V) for 13 weeks (50 mg/m(3)). In vitro experiments were done with original and extracted particles, as well as organic extracts of CB in DMSO. As positive controls, B[a]P (0.03 mu M) and a mixture of 16 PAHs (0.1 mu M) were used. Results: No leaching of PAHs was measured in saline or surfactant-containing saline. In vitro incubations with CB particles (30-300 mu g/cm(2)) revealed no adduct spots except for Sterling V. However, the spot was not concentration dependent and remains unidentified. Lung DNA from rats after inhalation of Printex 90 or Sterling V showed no spots related to PAH-DNA adduct formation compared to sham-exposed rats. Conclusion: The results suggest that PAHs are very tightly bound to these CBs. Only using organic extracts or particles of low-surface Sterling V, with high PAH content, PAHs may become available to form PAH-DNA adducts. However, the in vitro conditions showing this effect will not be encountered in vivo and renders this mechanism in particle-induced lung cancer at in vivo exposures highly unlikely. (c) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:157 / 167
页数:11
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