Basic fibroblast growth factor mediates angiogenic activity in early surgical wounds

被引:102
作者
Nissen, NN
Polverini, PJ
Gamelli, RL
DiPietro, LA
机构
[1] LOYOLA UNIV,MED CTR,DEPT SURG,BURN & SHOCK TRAUMA INST,MAYWOOD,IL 60153
[2] UNIV MICHIGAN,SCH DENT,LAB MOLEC PATHOL,ANN ARBOR,MI 48109
基金
美国国家卫生研究院;
关键词
D O I
10.1016/S0039-6060(96)80148-6
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background. Wound angiogenesis is believed to be initiated by the early rapid release of preformed growth factors such as basic fibroblast growth factor (bFGF). However, neither the angiogenic environment of early surgical wounds nor the potential contribution of bFGF to early surgical wound angiogenesis has been investigated. Methods. We collected surgical drain fluid (SDF) from closed suction drains 6 hours to 6 days after operation. SDF was tested for endothelial cell (EC) proliferative and chemotactic activity and for the capacity to stimulate angiogenesis in vivo in the rat corneal assay. bFGF levels of SDF were determined with enzyme-linked immunosorbent assay. Neutralizing antibody to bFGF was used to determine the contribution of bFGF to SDP activity. Results. The EC proliferative activity of SDF was maximal on postoperative day 0 (POD 0, 390% that of normal serum) and then fell by 41% on POD 1 and to near serum levels thereafter. SDF from PODs 0 and 1 also showed marked EC chemotactic activity and stimulated rapid formation of new vessels without signs of inflammation when implanted into rat corneas. The temporal appearance of bFGF in these exudates showed a pattern similar to EC proliferative activity, peaking on POD 0 at 854 pg/ml and decreasing 80% by POD 2. Neutralizing antibody to bFGF decreased the proliferative activity of SDF from PODs 0 and 1 to near serum levels and substantially decreased the chemotactic and the in vivo neovascular response to SDFs. Conclusions. Surgical wounds are characterized by a rapid and early angiogenic environment that is mediated in part by bFGF, suggesting that tissue or platelet stores of bFGF may help initiate wound repair.
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页码:457 / 465
页数:9
相关论文
共 48 条
[1]   NUCLEOTIDE-SEQUENCE OF A BOVINE CLONE ENCODING THE ANGIOGENIC PROTEIN, BASIC FIBROBLAST GROWTH-FACTOR [J].
ABRAHAM, JA ;
MERGIA, A ;
WHANG, JL ;
TUMOLO, A ;
FRIEDMAN, J ;
HJERRILD, KA ;
GOSPODAROWICZ, D ;
FIDDES, JC .
SCIENCE, 1986, 233 (4763) :545-548
[2]  
ARNOLD F, 1987, INT J MICROCIRC, V5, P381
[3]   ISOLATION OF A NONMITOGENIC ANGIOGENESIS FACTOR FROM WOUND FLUID [J].
BANDA, MJ ;
KNIGHTON, DR ;
HUNT, TK ;
WERB, Z .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (24) :7773-7777
[4]   BASIC FIBROBLAST GROWTH-FACTOR BINDS TO SUBENDOTHELIAL EXTRACELLULAR-MATRIX AND IS RELEASED BY HEPARITINASE AND HEPARIN-LIKE MOLECULES [J].
BASHKIN, P ;
DOCTROW, S ;
KLAGSBRUN, M ;
SVAHN, CM ;
FOLKMAN, J ;
VLODAVSKY, I .
BIOCHEMISTRY, 1989, 28 (04) :1737-1743
[5]   NEO-VASCULOGENIC ABILITY OF PROSTAGLANDINS, GROWTH-FACTORS, AND SYNTHETIC CHEMOATTRACTANTS [J].
BENEZRA, D .
AMERICAN JOURNAL OF OPHTHALMOLOGY, 1978, 86 (04) :455-461
[6]  
BENEZRA M, 1993, BLOOD, V81, P3324
[7]  
BROADLEY KN, 1989, LAB INVEST, V61, P571
[8]  
BRUNNER G, 1993, BLOOD, V81, P631
[9]  
BRUNNER G, 1994, BLOOD, V83, P2115
[10]   USE OF AN AQUEOUS SOLUBLE TETRAZOLIUM FORMAZAN ASSAY TO MEASURE VIABILITY AND PROLIFERATION OF LYMPHOKINE-DEPENDENT CELL-LINES [J].
BUTTKE, TM ;
MCCUBREY, JA ;
OWEN, TC .
JOURNAL OF IMMUNOLOGICAL METHODS, 1993, 157 (1-2) :233-240