Fluorescent properties of model chromophores of tyrosine-66 substituted mutants of Aequorea green fluorescent protein (GFP)

被引：143

作者：

Kojima, S

Ohkawa, H

Hirano, T

Maki, S

Niwa, H

Ohashi, M

Inouye, S

Tsuji, FI

机构：

[1] Univ Electrocommun, Dept Appl Phys & Chem, Chofu, Tokyo 182, Japan

[2] Chisso Corp, Yokohama Res Ctr, Kanazawa Ku, Yokohama, Kanagawa 236, Japan

[3] Univ Calif San Diego, Scripps Inst Oceanog, Marine Biol Res Div 0202, La Jolla, CA 92093 USA

来源：

TETRAHEDRON LETTERS | 1998年 / 39卷 / 29期

关键词：

Erlermeyer azlactone synthesis; fluorescence; mutation; proteins;

D O I：

10.1016/S0040-4039(98)01031-4

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

In an ethanol-glass marix at 77 K, model compounds of the chromophores of recombinant GFP-Y66F, GFP-Y66W, and GEP-Y66H exhibited fluorescence properties close to those of the corresponding mutant proteins. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

收藏

页码：5239 / 5242

页数：4

相关论文

共 26 条

[1] Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein [J].

Brejc, K ;

Sixma, TK ;

Kitts, PA ;

Kain, SR ;

Tsien, RY ;

Ormo, M ;

Remington, SJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) :2306-2311

[2]

BUCK JS, 1943, ORG SYNTH, V2, P55

[3] GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION [J].

CHALFIE, M ;

TU, Y ;

EUSKIRCHEN, G ;

WARD, WW ;

PRASHER, DC .

SCIENCE, 1994, 263 (5148) :802-805

[4] AMINO-ACID SEQUENCE OF THE CALCIUM-DEPENDENT PHOTOPROTEIN AEQUORIN [J].

CHARBONNEAU, H ;

WALSH, KA ;

MCCANN, RO ;

PRENDERGAST, FG ;

CORMIER, MJ ;

VANAMAN, TC .

BIOCHEMISTRY, 1985, 24 (24) :6762-6771

[5] CHEMICAL-STRUCTURE OF THE HEXAPEPTIDE CHROMOPHORE OF THE AEQUOREA GREEN-FLUORESCENT PROTEIN [J].

CODY, CW ;

PRASHER, DC ;

WESTLER, WM ;

PRENDERGAST, FG ;

WARD, WW .

BIOCHEMISTRY, 1993, 32 (05) :1212-1218

[6] UNDERSTANDING, IMPROVING AND USING GREEN FLUORESCENT PROTEINS [J].

CUBITT, AB ;

HEIM, R ;

ADAMS, SR ;

BOYD, AE ;

GROSS, LA ;

TSIEN, RY .

TRENDS IN BIOCHEMICAL SCIENCES, 1995, 20 (11) :448-455

[7] RED-SHIFTED EXCITATION MUTANTS OF THE GREEN FLUORESCENT PROTEIN [J].

DELAGRAVE, S ;

HAWTIN, RE ;

SILVA, CM ;

YANG, MM ;

YOUVAN, DC .

BIO-TECHNOLOGY, 1995, 13 (02) :151-154

[8] GREEN-FLUORESCENT PROTEIN MUTANTS WITH ALTERED FLUORESCENCE EXCITATION-SPECTRA [J].

EHRIG, T ;

OKANE, DJ ;

PRENDERGAST, FG .

FEBS LETTERS, 1995, 367 (02) :163-166

[9]

HARHASH AH, 1971, INDIAN J CHEM, V9, P789

[10] WAVELENGTH MUTATIONS AND POSTTRANSLATIONAL AUTOXIDATION OF GREEN FLUORESCENT PROTEIN [J].

HEIM, R ;

PRASHER, DC ;

TSIEN, RY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (26) :12501-12504