Cellulases and Xylanases Production by Penicillium echinulatum Grown on Sugar Cane Bagasse in Solid-State Fermentation

被引:50
作者
Camassola, Marli [1 ]
Dillon, Aldo J. P. [1 ]
机构
[1] Univ Caxias do Sul, Inst Biotechnol, BR-95070560 Caxias Do Sul, RS, Brazil
关键词
Solid-state fermentation; Cellulases; Xylanases; Sugar cane bagasse; Second-generation ethanol; ALPHA-AMYLASE PRODUCTION; TRICHODERMA-REESEI; SUBSTRATE FERMENTATION; CELLULOLYTIC ENZYMES; AGRICULTURAL WASTES; ASPERGILLUS-AWAMORI; WHEAT BRAN; CULTURE; HEMICELLULASES; PRETREATMENT;
D O I
10.1007/s12010-010-8967-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To investigate the production of cellulases and xylanases from Penicillium echinulatum 9A02S1, solid-state fermentation (SSF) was performed by using different ratios of sugar cane bagasse (SCB) and wheat bran (WB). The greatest filter paper activity obtained was 45.82 +/- 1.88 U gdm(-1) in a culture containing 6SCB/4WB on the third day. The greatest beta-glucosidase activities were 40.13 +/- 5.10 U gdm(-1) obtained on the third day for the 0SCB/10WB culture and 29.17 +/- 1.06 U gdm(-1) for the 2SCB/8WB culture. For endoglucanase, the greatest activities were 290.47 +/- 43.57 and 276.84 +/- 15.47 U gdm(-1), for the culture 6SCB/4WB on the fourth and fifth days of cultivation, respectively. The greatest xylanase activities were found on the third day for the cultures 6SCB/4WB (36.38 +/- 5.38 U gdm(-1)) and 4SCB/6WB (37.87 +/- 2.26 U gdm(-1)). In conclusion, the results presented in this article showed that it was possible to obtain large amounts of cellulases and xylanases enzymes using low-cost substrates, such as SCB and WB.
引用
收藏
页码:1889 / 1900
页数:12
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