The cytoplasmic zinc finger protein ZPR1 accumulates in the nucleolus of proliferating

被引:74
作者
Galcheva-Gargova, Z
Gangwani, L
Konstantinov, KN
Mikrut, M
Theroux, SJ
Enoch, T
Davis, RJ [1 ]
机构
[1] Univ Massachusetts, Sch Med, Howard Hughes Med Inst, Worcester, MA 01605 USA
[2] Univ Massachusetts, Sch Med, Dept Biochem & Mol Biol, Program Mol Med, Worcester, MA 01605 USA
[3] Assumption Coll, Dept Biol, Worcester, MA 01615 USA
[4] Scripps Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA
[5] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA
关键词
D O I
10.1091/mbc.9.10.2963
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The zinc finger protein ZPR1 translocates from the cytoplasm to the nucleus after treatment of cells with mitogens. The function of nuclear ZPR1 has not been defined. Here we demonstrate that ZPR1 accumulates in the nucleolus of proliferating cells. The role of ZPR1 was examined using a gene disruption strategy. Cells lacking ZPR1 are not viable. Biochemical analysis demonstrated that the loss of ZPR1 caused disruption of nucleolar function, including preribosomal RNA expression. These data establish ZPR1 as an essential protein that is required for normal nucleolar function in proliferating cells.
引用
收藏
页码:2963 / 2971
页数:9
相关论文
共 16 条
  • [1] ABELSON HT, 1975, HDB EXP PHARMAKOL, V38, P571
  • [2] HUMAN AUTOANTIBODY TO A NOVEL PROTEIN OF THE NUCLEAR COILED BODY - IMMUNOLOGICAL CHARACTERIZATION AND CDNA CLONING OF P80-COILIN
    ANDRADE, LEC
    CHAN, EKL
    RASKA, I
    PEEBLES, CL
    ROOS, G
    TAN, EM
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1991, 173 (06) : 1407 - 1419
  • [3] FACTOR REQUIRED FOR MAMMALIAN SPLICEOSOME ASSEMBLY IS LOCALIZED TO DISCRETE REGIONS IN THE NUCLEUS
    FU, XD
    MANIATIS, T
    [J]. NATURE, 1990, 343 (6257) : 437 - 441
  • [4] Binding of zinc finger protein ZPR1 to the epidermal growth factor receptor
    GalchevaGargova, Z
    Konstantinov, KN
    Wu, IH
    Klier, FG
    Barrett, T
    Davis, RJ
    [J]. SCIENCE, 1996, 272 (5269) : 1797 - 1802
  • [5] NUCLEOLAR NECKLACES IN CHICK-EMBRYO FIBROBLAST CELLS .2. MICROSCOPE OBSERVATIONS OF EFFECT OF ADENOSINE ANALOGUES ON NUCLEOLAR NECKLACE FORMATION
    GRANICK, D
    [J]. JOURNAL OF CELL BIOLOGY, 1975, 65 (02) : 418 - 427
  • [6] Oncoprotein networks
    Hunter, T
    [J]. CELL, 1997, 88 (03) : 333 - 346
  • [7] KOTZIN BL, 1984, J IMMUNOL, V133, P2554
  • [8] MORENO S, 1991, METHOD ENZYMOL, V194, P795
  • [9] FIBRILLARIN - A NEW PROTEIN OF THE NUCLEOLUS IDENTIFIED BY AUTOIMMUNE SERA
    OCHS, RL
    LISCHWE, MA
    SPOHN, WH
    BUSCH, H
    [J]. BIOLOGY OF THE CELL, 1985, 54 (02) : 123 - 133
  • [10] MONOCLONAL AUTOANTIBODY FROM A (NEW-ZEALAND BLACK X NEW-ZEALAND WHITE)F1 MOUSE AND SOME HUMAN SCLERODERMA SERA TARGET AN MR-34,000 NUCLEOLAR PROTEIN OF THE U3 RNP PARTICLE
    REIMER, G
    POLLARD, KM
    PENNING, CA
    OCHS, RL
    LISCHWE, MA
    BUSCH, H
    TAN, EM
    [J]. ARTHRITIS AND RHEUMATISM, 1987, 30 (07): : 793 - 800