Enzymatic oxidation of H2 in atmospheric O2:: The electrochemistry of energy generation from trace H2 by aerobic microorganisms

被引：42

作者：

Cracknell, James A. ^{[1
]}

Vincent, Kylie A. ^{[1
]}

Ludwig, Marcus ^{[2
]}

Lenz, Oliver ^{[2
]}

Friedrich, Baerbel ^{[2
]}

Armstrong, Fraser A. ^{[1
]}

机构：

[1] Univ Oxford, Inorgan Chem Lab, Oxford OX1 3QR, England

[2] Humboldt Univ, Inst Biol Mikrobiol, D-10115 Berlin, Germany

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1021/ja078299+

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Certain microoganisims such as Ratsiona spp. can metabolize trace H-2 in air because they express an unusual O-2-tolerent [NiFe)-hydrogenase. Using protein film voltammetry methods we demonstrate the first quantitative measurements of the oxidation. by a purified enzyme of physiologically relevant levels of H-2 (below 10 ppm) in the presence of atmospheric O-2.

引用

页码：424 / +

页数：3

共 12 条

[1] [NiFe]-hydrogenases of Ralstonia eutropha H16:: Modular enzymes for oxygen-tolerant biological hydrogen oxidation [J].

Burgdorf, T ;

Lenz, O ;

Buhrke, T ;

van der Linden, E ;

Jones, AK ;

Albracht, SPJ ;

Friedrich, B .

JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY, 2005, 10 (2-4) :181-196

[2]

CONRAD R, 1988, ADV MICROB ECOL, V10, P231

[3] Soil microorganisms as controllers of atmospheric trace gases (H-2, CO, CH4, OCS, N2O, and NO) [J].

Conrad, R .

MICROBIOLOGICAL REVIEWS, 1996, 60 (04) :609-+

[4] Structure/function relationships of [NiFe]- and [FeFe]-hydrogenases [J].

Fontecilla-Camps, Juan C. ;

Volbeda, Anne ;

Cavazza, Christine ;

Nicolet, Yvain .

CHEMICAL REVIEWS, 2007, 107 (10) :4273-4303

[5] Direct comparison of the electrocatalytic oxidation of hydrogen by an enzyme and a platinum catalyst [J].

Jones, AK ;

Sillery, E ;

Albracht, SPJ ;

Armstrong, FA .

CHEMICAL COMMUNICATIONS, 2002, (08) :866-867

[6] Inhibition and aerobic inactivation kinetics of Desulfovibrio fructosovorans NiFe hydrogenase studied by protein film voltammetry [J].

Léger, C ;

Dementin, S ;

Bertrand, P ;

Rousset, M ;

Guigliarelli, B .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (38) :12162-12172

[7] Occurrence, classification, and biological function of hydrogenases: An overview [J].

Vignais, Paulette M. ;

Billoud, Bernard .

CHEMICAL REVIEWS, 2007, 107 (10) :4206-4272

[8] Electrochemical definitions of O2 sensitivity and oxidative inactivation in hydrogenases [J].

Vincent, KA ;

Parkin, A ;

Lenz, O ;

Albracht, SPJ ;

Fontecilla-Camps, JC ;

Cammack, R ;

Friedrich, B ;

Armstrong, FA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (51) :18179-18189

[9] Electrocatalytic hydrogen oxidation by an enzyme at high carbon monoxide or oxygen levels [J].

Vincent, KA ;

Cracknell, JA ;

Lenz, O ;

Zebger, I ;

Friedrich, B ;

Armstrong, FA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (47) :16951-16954

[10] Investigating and exploiting the electrocatalytic properties of hydrogenases [J].

Vincent, Kylie A. ;

Parkin, Alison ;

Armstrong, Fraser A. .

CHEMICAL REVIEWS, 2007, 107 (10) :4366-4413

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