Effect of ischemia-reperfusion on bile canalicular F-actin microfilaments in hepatocytes of human liver allograft -: Image analysis by confocal laser scanning microscopy

被引：25

作者：

Benkoël, L

Dodero, F

Hardwigsen, J

Campan, P

Botta-Fridlund, D

Lombardo, D

Le Treut, YP

Chamlian, A

机构：

[1] Fac Med Marseille, INSERM, U559, Lab Pathol Cellulaire & Metab Foie, F-13385 Marseille 05, France

[2] Hop Conception, Unite Transplantat Hepat, F-13385 Marseille, France

[3] Hop Conception, Serv Hepatogastroenterol, F-13385 Marseille 05, France

来源：

DIGESTIVE DISEASES AND SCIENCES | 2001年 / 46卷 / 08期

关键词：

human liver allografts; ischemia-reperfusion; F-actin; confocal laser scanning microscopy;

D O I：

10.1023/A:1010693218680

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

We studied and quantified the effect of ischemia-reperfusion on hepatic F-actin on bile canalicular and basolateral membranes in human liver allografts by means of confocal laser scanning microscopy imaging. The phalloidin-FITC staining of F-actin was normal in liver hepatocytes before reperfusion but decreased significantly after reperfusion (by 25% of controls). These results indicate that hepatic F-actin alteration is produced during the reperfusion phase. This modification, probably induced by reactive oxygen species, could impair bile canalicular contraction and tight junction permeability and consequently bile secretion in the postoperative period.

引用

页码：1663 / 1667

页数：5

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