Caenorhabditis elegans expresses a functional phytochelatin synthase

被引:115
作者
Clemens, S
Schroeder, JI
Degenkolb, T
机构
[1] Leibniz Inst Plant Biochem, D-06120 Halle, Germany
[2] Univ Calif San Diego, Div Biol, Cell & Dev Biol Sect, La Jolla, CA 92093 USA
[3] Univ Calif San Diego, Ctr Mol Genet, La Jolla, CA 92093 USA
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 2001年 / 268卷 / 13期
关键词
metal tolerance; metal homeostasis; phytochelatins;
D O I
10.1046/j.1432-1327.2001.02293.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The formation of phytochelatins, small metal-binding glutathione-derived peptides, is one of the well-studied responses of plants to toxic metal exposure. Phytochelatins have also been detected in some fungi and some marine diatoms. Genes encoding phytochelatin synthases (PCS) have recently been cloned from Arabidopsis, wheat and Schizosaccharomyces pombe. Surprisingly, database searches revealed the presence of a homologous gene in the Caenorhabditis elegans genome, DDBJ/EMBL/GenBank accession no. 266513. Here we show that C. elegans indeed expresses a gene coding for a functional phytochelatin synthase. CePCS complements the Cd2+ sensitivity of a Schizosaccharomyces pombe PCS knock-out strain and confers phytochelatin synthase activity to these cells. Thus, phytochelatins may play a role for metal homeostasis also in certain animals.
引用
收藏
页码:3640 / 3643
页数:4
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