A transposon-based activation-tagging population in Arabidopsis thaliana (TAMARA) and its application in the identification of dominant developmental and metabolic mutations

被引：24

作者：

Schneider, A

Kirch, T

Gigolashvili, T

Mock, HP

Sonnewald, U

Simon, R

Flügge, UI

Werr, W

机构：

[1] Univ Dusseldorf, Genet Inst, D-40225 Dusseldorf, Germany

[2] Univ Erlangen Nurnberg, Lehrstuhl Biochem, D-91058 Erlangen, Germany

[3] Inst Pflanzengenet & Kulturpflanzenforsch, D-06466 Gatersleben, Germany

[4] Univ Cologne, Inst Entwicklungsbiol, D-50931 Cologne, Germany

[5] Univ Cologne, Inst Bot, D-50931 Cologne, Germany

来源：

FEBS LETTERS | 2005年 / 579卷 / 21期

关键词：

transposon; activation tagging; dominant mutation; shoot development; phenylpropanoid metabolism;

D O I：

10.1016/j.febslet.2005.07.030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A population of 9471 stable activation-tagged lines was generated by transposable element mediated activation tagging mutagenesis in Arabidopsis (TAMARA) using the maize EnlSpm transposon system. Based on DNA gel blot and flanking sequence analysis, this population contains approximately 6000 independent transposon insertions. A greenhouse-based screen identified six dominant or semi-dominant activation tagged mutants with obvious developmental alterations, among these a new pistillata mutant allele. In addition, a subset of 1500 lines was screened by a HPLC based high-throughput method for dominant activation tagged mutants with enhanced contents of phenolic compounds. One dominant activation tagged mutant (hpc1-1D) was isolated showing accumulation of a particular compound due to the upregulation of an R2R3-MYB transcription factor. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4622 / 4628

页数：7

共 39 条

[1] A 2-ELEMENT ENHANCER-INHIBITOR TRANSPOSON SYSTEM IN ARABIDOPSIS-THALIANA [J].