A versatile plasmid biosensor useful to identify quorum sensing LuxR-family orphans in bacterial strains

被引：9

作者：

Steindler, Laura ^{[1
]}

Devescovi, Giulia ^{[1
]}

Subramoni, Sujatha ^{[1
]}

Venturi, Vittorio ^{[1
,2
]}

机构：

[1] Int Ctr Genet Engn & Biotechnol, Bacteriol Grp, I-34012 Trieste, Italy

[2] Int Ctr Genet Engn & Biotechnol, Plant Bacteriol Grp, Treviso, Italy

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2008年 / 73卷 / 03期

关键词：

quorum sensing; N-acyl homoserine lactone; LuxR; biosensor; plasmid;

D O I：

10.1016/j.mimet.2008.02.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Eight luxl-family gene promoters (luxI, cvil, ahll, rhll, cepl, phzl, tral and ppul) were cloned in tandem, upstream a promoterless lacZ gene in a promoter probe vector yielding pMULTIAHLPROM. This unique construct is useful in determining whether a bacterial strain not producing N-acyl homoserine lactone signal molecules (AHLs) possesses orphan LuxR type proteins able to respond to AHLs and activate transcription from quorum sensing target genes. Using pMULTIAHLPROM, it was demonstrated that Enterobacter aerogenes possibly contains a LuxR-family orphan able to activate luxl-family promoters independently from AHLs. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：273 / 275

页数：3

共 21 条

[1] Quorum-sensing system and stationary-phase sigma factor (rpoS) of the onion pathogen Burkholderia cepacia genomovar I type strain, ATCC 25416 [J].

Aguilar, C ;

Bertani, I ;

Venturi, V .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (03) :1739-1747

[2] Plant responses to bacterial quorum sensing signals [J].

Bauer, WD ;

Mathesius, U .

CURRENT OPINION IN PLANT BIOLOGY, 2004, 7 (04) :429-433

[3] Regulation of the N-acyl homoserine lactone-dependent quorum-sensing system in rhizosphere Pseudomonas putida WCS358 and cross-talk with the stationary-phase RpoS sigma factor and the global regulator GacA [J].

Bertani, I ;

Venturi, V .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (09) :5493-5502

[4] Bacterial small-molecule signaling pathways [J].

Camilli, A ;

Bassler, BL .

SCIENCE, 2006, 311 (5764) :1113-1116

[5] Oryza sativa rice plants contain molecules that activate different quorum-sensing N-acyl homoserine lactone biosensors and are sensitive to the specific AiiA lactonase [J].

Degrassi, Giuliano ;

Devescovi, Giulia ;

Solis, Renando ;

Steindler, Laura ;

Venturi, Vittorio .

FEMS MICROBIOLOGY LETTERS, 2007, 269 (02) :213-220

[6]

Farrand SK, 2002, METHOD ENZYMOL, V358, P452

[7] A LuxR homologue of Xanthomonas oryzae pv. oryzae is required for optimal rice virulence [J].

Ferluga, Sara ;

Bigirimana, Joseph ;

Hofte, Monica ;

Venturi, Vittorio .

MOLECULAR PLANT PATHOLOGY, 2007, 8 (04) :529-538

[8] The QscR quorum-sensing regulon of Pseudomonas aeruginosa:: an orphan claims its identity [J].

Fuqua, C .

JOURNAL OF BACTERIOLOGY, 2006, 188 (09) :3169-3171

[9]

GEELS FP, 1983, PHYTOPATHOL Z, V108, P207

[10] Synthesis of N-acyl homoserine lactone analogues reveals strong activators of SdiA, the Salmonella enterica serovar Typhimurium LuxR homologue [J].

Janssens, Joost C. A. ;

Metzger, Kristine ;

Daniels, Ruth ;

Ptacek, Dave ;

Verhoeven, Tine ;

Habel, Lothar W. ;

Vanderleyden, Jos ;

De Vos, Dirk E. ;

De Keersmaecker, Sigrid C. J. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (02) :535-544

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