A recurring theme in protein engineering: the design, stability and folding of repeat proteins

被引：109

作者：

Main, ERG

Lowe, AR

Mochrie, SGJ

Jackson, SE

Regan, L

机构：

[1] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

[2] Yale Univ, Dept Chem, New Haven, CT 06520 USA

[3] Univ Cambridge, Chem Lab, Cambridge CB2 1EW, England

[4] Univ Sussex, Dept Chem, Chem Biol Unit, Brighton BN1 9QG, E Sussex, England

[5] MRC, Canc Cell Unit, Hutchison MRC Res Ctr, Cambridge CB2 2XZ, England

[6] Yale Univ, Dept Phys, New Haven, CT 06520 USA

[7] Yale Univ, Dept Appl Phys, New Haven, CT 06520 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2005年 / 15卷 / 04期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/j.sbi.2005.07.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Repeat proteins are ubiquitous and are involved in a myriad of essential processes. They are typically non-globular structures that act as diverse scaffolds for the mediation of protein-protein interactions. These excitingly different structures, which arise from tandem arrays of a repeated structural motif, have generated significant interest with respect to protein engineering and design. Recent advances have been made in the design and characterisation of repeat proteins. The highlights include re-engineering of binding specificity, quantitative models of repeat protein stability and kinetic studies of repeat protein folding.

引用

收藏

页码：464 / 471

页数：8

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