Statistical methods for comparing comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry results:: Metabolomic analysis of mouse tissue extracts

被引：109

作者：

Shellie, RA

Welthagen, W

Zrostliková, J

Spranger, J

Ristow, M

Fiehn, O

Zimmermann, R ^{[1
]}

机构：

[1] GSF Res Ctr, Inst Ecol Chem, Oberschleissheim, Germany

[2] Max Planck Inst Mol Plant Physiol, Golm, Germany

[3] Univ Augsburg, D-8900 Augsburg, Germany

[4] Leco Instruments, Prague, Czech Republic

[5] German Inst Human Nutr, Rehbrucke, Germany

[6] Charite, Berlin, Germany

[7] Univ Calif Davis, UC Davis Genome Ctr, Davis, CA 95616 USA

[8] BIfA, Bavarian Inst Appl Environm Res & Technol, Augsburg, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1086卷 / 1-2期

关键词：

gas chromatography; comprehensive two-dimensional; mass spectrometry; time-of-flight; metabolomics; data processing;

D O I：

10.1016/j.chroma.2005.05.088

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The potential for the utilization of GC x GC-time-of-flight (TOF) MS for high-resolution metabolomics studies is discussed, with the implementation of some statistical comparisons for biomarker detection. Metabolite profiles from NZO obese mice versus BL/6 control mice are compared and contrasted using a number of chromatogram comparison routines, including direct chromatogram comparisons, chromatogram subtraction and averaging routines, as well as a method for generating relative weighted peak surface difference chromatograms, and a more conventional Student's t-test statistical approach. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：83 / 90

页数：8

共 21 条

[1] Discrimination of three tobacco types (Burley, Virginia and Oriental) by pyrolysis single-photon ionisation-time-of-flight mass spectrometry and advanced statistical methods [J].