Diagnosis of botulism types C and D in cattle by a monoclonal antibody-based sandwich ELISA

被引：12

作者：

Brooks, C. E. ^{[1
]}

Clarke, H. J. ^{[1
]}

Graham, D. A. ^{[1
]}

Ball, H. J. ^{[1
]}

机构：

[1] Agri Food & Biosci Inst No Ireland, Vet Sci Div, Belfast BT4 3SD, Antrim, North Ireland

来源：

VETERINARY RECORD | 2011年 / 168卷 / 17期

关键词：

CLOSTRIDIUM-BOTULINUM; SENSITIVITY; TOXIN;

D O I：

10.1136/vr.c7432

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

This study was undertaken to evaluate two monoclonal antibody-based sandwich ELISAs (sELISAs) for the detection of Clostridium botulinum neurotoxins (BoNTs) types C and D from culture-enriched intestinal content samples from cattle. To validate the diagnostic significance of the presence of cultivable, toxin-producing C botulinum in the intestines of cattle, samples from both suspect and non-suspect botulism cases were examined. BoNT was detected by both sELISAs in a greater number of suspect animals than by direct testing of uncultured samples by mouse bioassay. One sELISA detected two BoNT C and one BoNT Group III mosaic isoform in three animals that were missed by the other, and both sELISAs failed to identify samples from two mouse bioassay-positive BoNT C animals. BoNT D was also detected in one non-suspect sample by one of the sELISAs.

引用

页数：5

共 18 条

[1] STATISTICS NOTES - DIAGNOSTIC-TESTS-1 - SENSITIVITY AND SPECIFICITY .3. [J].

ALTMAN, DG ;

BLAND, JM .

BRITISH MEDICAL JOURNAL, 1994, 308 (6943) :1552-1552

[2] AN OUTBREAK OF BOTULISM IN BROILER CHICKENS [J].

BLANDFORD, TB ;

ROBERTS, TA .

VETERINARY RECORD, 1970, 87 (09) :258-+

[3] Culture enrichment assists the diagnosis of cattle botulism by a monoclonal antibody based sandwich ELISA [J].

Brooks, C. E. ;

Clarke, H. J. ;

Finlay, D. A. ;

McConnell, W. ;

Graham, D. A. ;

Ball, H. J. .

VETERINARY MICROBIOLOGY, 2010, 144 (1-2) :226-230

[4]

CDC, 1998, Botulism in the United States, 1899-1996 Handbook for Epidemiologists, Clinicians, and Laboratory Workers

[5]

CLARKE HJ, 2009, CLOSTRIDIA IMPACT GE

[6] COPROEXAMINATION FOR BOTULINAL TOXIN AND CLOSTRIDIUM-BOTULINUM - NEW PROCEDURE FOR LABORATORY DIAGNOSIS OF BOTULISM [J].

DOWELL, VR ;

MCCROSKEY, LM ;

HATHEWAY, CL ;

LOMBARD, GL ;

HUGHES, JM ;

MERSON, MH .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1977, 238 (17) :1829-1832

[7] Detection of Clostridium botulinum type A toxin in a cow in Northern Ireland [J].

Hartley, H. M. ;

Clarke, H. J. ;

Graham, D. A. ;

Ball, H. J. ;

Malone, F. E. ;

Beggs, N. W. R. ;

Roest, H. J. .

VETERINARY RECORD, 2009, 165 (18) :544-544

[8] THERMAL SENSITIVITY OF CLOSTRIDIUM-BOTULINUM TYPE-C TOXIN [J].

HUBALEK, Z ;

HALOUZKA, J .

EPIDEMIOLOGY AND INFECTION, 1988, 101 (02) :321-325

[9] Mosaic structures of neurotoxins produced from Clostridium botulinum types C and D organisms [J].

Moriishi, K ;

Koura, M ;

Abe, N ;

Fujii, N ;

Fujinaga, Y ;

Inoue, K ;

Ogumad, K .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1996, 1307 (02) :123-126

[10] EXAMINATION OF BOVINE FECES FOR THE ISOLATION AND IDENTIFICATION OF CLOSTRIDIUM SPECIES [J].

PRINCEWELL, TJT ;

AGBA, MI .

JOURNAL OF APPLIED BACTERIOLOGY, 1982, 52 (01) :97-102

← 1 2 →