Bisphenol-A affects spermatogenesis in the adult rat even at a low dose

被引：124

作者：

Sakaue, M

Ohsako, S

Ishimura, R

Kurosawa, S

Kurohmaru, M

Hayashi, Y

Aoki, Y

Yonemoto, J

Tohyama, C

机构：

[1] Univ Tokyo, Natl Inst Environm Studies, Environm Hlth Sci Div, Tsukuba, Ibaraki 3058506, Japan

[2] Univ Tokyo, Natl Inst Environm Studies, Endocrine Disrupters & Dioxin Res Project, Tsukuba, Ibaraki, Japan

[3] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Anat, Tsukuba, Ibaraki, Japan

来源：

JOURNAL OF OCCUPATIONAL HEALTH | 2001年 / 43卷 / 04期

关键词：

bisphenol-A; low dose; adult rat; testis; spermatogenesis; daily sperm production; testicular weight; 2D-PAGE;

D O I：

10.1539/joh.43.185

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Bisphenol-A (BPA), a xenobiotic estrogenic compound widely used as a plastics monomer, has been suspected to have a so-called low dose effect on the reproductive system when administered transplacentally. In the present study, we investigated possible low-dose effects of BPA on spermatogenesis in adult rats. Male rats (13 weeks old; W13) were administrated a daily oral dose of BPA, ranging from 2 ng to 200 mg/kg, for 6 days and examined for testicular weight (TW) and daily sperm production (DSP) at W14 and W18. A BPA dose as low as 20 mug/kg tended to decrease TW and significantly reduced both DSP and the efficiency of spermatogenesis (DSP per gram testis) at W18, showing that BPA suppressed a normal increase in DSP and TW from W13 to W18. A single administration of 20 mug BPA/kg to W13 rats affected the intensity or mobility of several protein spots in the testicular cytosol fraction as shown by two-dimensional gel electrophoresis analysis. The present study showed that BPA at a low dose affects spermatogenesis in the adult rat.

引用

页码：185 / 190

页数：6

共 29 条

[1] A case of a laboratory animal feed with high estrogenic activity and its impact on in vivo responses to exogenously administered estrogens -: Commentary [J].