Preparation and characterization of multilayered hydroxyapatite/silk fibroin film

被引:48
作者
Kino, Rikako
Ikorna, Toshiyuki
Yunoki, Shunji
Nagai, Nobuhiro
Tanaka, Junzo
Asakura, Tetsuo
Munekata, Masanobu
机构
[1] Hokkaido Univ, Fac Engn, Grad Sch Engn, Kita Ku, Sapporo, Hokkaido 0608628, Japan
[2] Hokkaido Univ, CRIS, Div Frontier Res, Kita Ku, Sapporo, Hokkaido 0010021, Japan
[3] Natl Inst Mat Sci, Biomat Ctr, Tsukuba, Ibaraki 3050044, Japan
[4] Tokyo Univ Agr & Technol, Dept Biotechnol, Tokyo 1848588, Japan
关键词
silk fibroin; hydroxyapatite; composite; T-peel test;
D O I
10.1263/jbb.103.514
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We prepared multilayered films consisting of silk fibroin (SF) and hydroxyapatite (HAp) by alternating lamination using untreated SF and HAp-deposited SIT films. Untreated SF films were prepared from a regenerated SIT solution by air drying. HAp-deposited SF films were prepared by soaking methanol-treated SF films containing > 5 wt% CaCl2 in a simulated body fluid with the ion concentration 1.5-fold higher than that of the standard one. The multilayered HAp/SF films had HAp layers with approximate thicknesses of 3-5 mu m and SIT layers with thicknesses of 4070 pm. The bonding strength between the SF and HAp layers was significantly affected by temperature and compression time under the lamination method. The optimal conditions for achieving the maximum T-peel strength and beta-sheet contents were determined to be 130 degrees C for 4 min. The Young's modulus of the multilayered films (133.4 MPa) was higher than that of the films consisting of SF alone (92.5 MPa) under swollen conditions. The biocompatibility of the HAp-deposited SIT films was analyzed by culturing of osteoblasts (MC3T3-E1) on a film. The results indicate that HAp-deposited SIT films and SIT films show similar degrees of cell adhesion and alkaline phosphatase activities.
引用
收藏
页码:514 / 520
页数:7
相关论文
共 46 条
[1]   Silk-based biomaterials [J].
Altman, GH ;
Diaz, F ;
Jakuba, C ;
Calabro, T ;
Horan, RL ;
Chen, JS ;
Lu, H ;
Richmond, J ;
Kaplan, DL .
BIOMATERIALS, 2003, 24 (03) :401-416
[2]   Silk matrix for tissue engineered anterior cruciate ligaments [J].
Altman, GH ;
Horan, RL ;
Lu, HH ;
Moreau, J ;
Martin, I ;
Richmond, JC ;
Kaplan, DL .
BIOMATERIALS, 2002, 23 (20) :4131-4141
[3]  
Bartee B K, 1995, J Oral Implantol, V21, P88
[4]   Nanocomposites of hydroxyapatite with aspartic acid and glutamic acid and their interaction with osteoblast-like cells [J].
Boanini, Elisa ;
Torricelli, Paola ;
Gazzano, Massimo ;
Giardino, Roberto ;
Bigi, Adriana .
BIOMATERIALS, 2006, 27 (25) :4428-4433
[5]   Surface modification of poly (D,L-lactic acid) with chitosan and its effects on the culture of osteoblasts in vitro [J].
Cai, KY ;
Yao, KD ;
Cui, YL ;
Lin, SB ;
Yang, ZM ;
Li, XQ ;
Xie, HQ ;
Qing, TW ;
Luo, J .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 2002, 60 (03) :398-404
[6]   De novo engineering of reticular connective tissue in vivo by silk fibroin nonwoven materials [J].
Dal Pra, I ;
Freddi, G ;
Minic, J ;
Chiarini, A ;
Armato, U .
BIOMATERIALS, 2005, 26 (14) :1987-1999
[7]   Nano-scaled hydroxyapatite/polymer composite I.: Coating of sintered hydroxyapatite particles on poly(γ-methacryloxypropyl trimethoxysilane)-grafted silk fibroin fibers through chemical bonding [J].
Furuzono, T ;
Kishida, A ;
Tanaka, J .
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2004, 15 (01) :19-23
[8]  
Furuzono T, 1999, J APPL POLYM SCI, V73, P2541, DOI 10.1002/(SICI)1097-4628(19990919)73:12<2541::AID-APP23>3.0.CO
[9]  
2-5
[10]   Chemical modification of silk fibroin with 2-methacryloyloxyethyl phosphorylcholine. II. Craft-polymerization onto fabric through 2-methacryloyloxyethyl isocyanate and interaction between fabric and platelets [J].
Furuzono, T ;
Ishihara, K ;
Nakabayashi, N ;
Tamada, Y .
BIOMATERIALS, 2000, 21 (04) :327-333