Loss of cytochrome c Fe(III)/Fe(II) redox couple in ionic liquids

被引：28

作者：

Compton, DL ^{[1
]}

Laszlo, JA ^{[1
]}

机构：

[1] USDA ARS, Natl Ctr Agr Utilizat Res, New Crops & Proc Technol Res Unit, Peoria, IL 61604 USA

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2003年 / 553卷

关键词：

cyclic voltammetry; cytochrome c; ionic liquids; self assembled monolayer;

D O I：

10.1016/S0022-0728(03)00324-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The electrochemical behavior of cytochrome c (cyt-c) adsorbed to gold electrodes through mercaptoalkanoic acid/ mercaptoalcohol monolayers was investigated in the 1-butyl-3-methylimidazolium salts of bis(trifluoromethylsulfonyl)imide and hexafluorophosphate. Cyt-c does not retain its Fe(III)/Fe(II) redox activity in dry ionic liquids (ILs); however, the redox signal of the modified electrodes can be reconstituted upon subsequent treatment with aqueous buffer. Water saturated ILs, 1.4 wt.%, failed to support the redox activity of the adsorbed cyt-c; therefore, the ILs' deleterious effects on the redox behavior of the cyt-c modified electrodes cannot be attributed solely to the stripping of essential water from the enzyme's polypeptide lattice. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：187 / 190

页数：4

共 18 条

[1] Hydrophobic, highly conductive ambient-temperature molten salts [J].

Bonhote, P ;

Dias, AP ;

Papageorgiou, N ;

Kalyanasundaram, K ;

Gratzel, M .

INORGANIC CHEMISTRY, 1996, 35 (05) :1168-1178

[2] Voltammetric peak broadening for cytochrome c/alkanethiolate monolayer structures: Dispersion of formal potentials [J].

Clark, RA ;

Bowden, EF .

LANGMUIR, 1997, 13 (03) :559-565

[3] Direct electrochemical reduction of hemin in imidazolium-based ionic liquids [J].

Compton, DL ;

Laszlo, JA .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2002, 520 (1-2) :71-78

[4] Controlling interfacial electron-transfer kinetics of cytochrome c with mixed self-assembled monolayers [J].

El Kasmi, A ;

Wallace, JM ;

Bowden, EF ;

Binet, SM ;

Linderman, RJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (01) :225-226

[5] Superoxide sensor based on cytochrome c immobilized on mixed-thiol SAM with a new calibration method [J].

Ge, B ;

Lisdat, F .

ANALYTICA CHIMICA ACTA, 2002, 454 (01) :53-64

[6] ENZYME FUNCTION IN ORGANIC-SOLVENTS [J].

GUPTA, MN .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 203 (1-2) :25-32

[7] Comparison of peroxidase activities of hemin, cytochrome c and microperoxidase-11 in molecular solvents and imidazolium-based ionic liquids [J].

Laszlo, JA ;

Compton, DL .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2002, 18 (1-3) :109-120

[8] α-chymotrypsin catalysis in imidazolium-based ionic liquids [J].

Laszlo, JA ;

Compton, DL .

BIOTECHNOLOGY AND BIOENGINEERING, 2001, 75 (02) :181-186

[9] Novel electrochemical studies of ionic liquids [J].

Quinn, BM ;

Ding, ZF ;

Moulton, R ;

Bard, AJ .

LANGMUIR, 2002, 18 (05) :1734-1742

[10] Conformational and redox equilibria and dynamics of cytochrome c immobilized on electrodes via hydrophobic interactions [J].

Rivas, L ;

Murgida, DH ;

Hildebrandt, P .

JOURNAL OF PHYSICAL CHEMISTRY B, 2002, 106 (18) :4823-4830

← 1 2 →