Rapid detection of West Nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCR assay

被引：869

作者：

Lanciotti, RS ^{[1
]}

Kerst, AJ ^{[1
]}

Nasci, RS ^{[1
]}

Godsey, MS ^{[1
]}

Mitchell, CJ ^{[1
]}

Savage, HM ^{[1
]}

Komar, N ^{[1
]}

Panella, NA ^{[1
]}

Allen, BC ^{[1
]}

Volpe, KE ^{[1
]}

Davis, BS ^{[1
]}

Roehrig, JT ^{[1
]}

机构：

[1] CDC, Div Vector Borne Infect Dis, Natl Ctr Infect Dis, Ft Collins, CO 80521 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 11期

关键词：

D O I：

10.1128/JCM.38.11.4066-4071.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

The authors report on the development and application of a rapid TaqMan assay for the detection of West Nile (WN) virus in a variety of human clinical specimens and field-collected specimens. Oligonucleotide primers and FAM- and TAMRA-labeled WN virus-specific probes were designed by using the nucleotide sequence of the New York 1999 WN virus isolate. The TaqMan assay was compared to a traditional reverse transcriptase (RT)-PCR assay and to virus isolation in Vero cells with a large number (approximate to 5500) of specimens obtained from humans (serum, cerebrospinal fluid, and brain tissue), field-collected mosquitoes, and avian tissue samples. The TaqMan assay was specific for WN virus and demonstrated a greater sensitivity than the traditional RT-PCR method and correctly identified WN virus in 100% of the culture-positive mosquito pools and 98% of the culture-positive avian tissue samples. The assay should be of utility in the diagnostic laboratory to complement existing human diagnostic testing and as a tool to conduct WN virus surveillance in the United States.

引用

页码：4066 / 4071

页数：6

共 17 条

[1]

Isolation of West Nile virus from mosquitoes, crows, and a Cooper's hawk in Connecticut [J].

Anderson, JF ;

Andreadis, TG ;

Vossbrinck, CR ;

Tirrell, S ;

Wakem, EM ;

French, RA ;

Garmendia, AE ;

Van Kruiningen, HJ .

SCIENCE, 1999, 286 (5448) :2331-2333

[2]

Beaty BJ., 1989, Diagnostic procedures for viral, rickettsial and chlamydial infections, P797

[3]

Centers for Disease Control and Prevention (CDC), 1999, MMWR Morb Mortal Wkly Rep, V48, P845

[4]

Hayes C. G., 1989, The arboviruses: epidemiology and ecology. Volume V., P59

[5]

5′ nuclease PCR assay to detect Yersinia pestis [J].

Higgins, JA ;

Ezzell, J ;

Hinnebusch, BJ ;

Shipley, M ;

Henchal, EA ;

Ibrahim, MS .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (08) :2284-2288

[6]

West Nile viral encephalitis [J].

Komar, N .

REVUE SCIENTIFIQUE ET TECHNIQUE-OFFICE INTERNATIONAL DES EPIZOOTIES, 2000, 19 (01) :166-176

[7]

Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States [J].

Lanciotti, RS ;

Roehrig, JT ;

Deubel, V ;

Smith, J ;

Parker, M ;

Steele, K ;

Crise, B ;

Volpe, KE ;

Crabtree, MB ;

Scherret, JH ;

Hall, RA ;

MacKenzie, JS ;

Cropp, CB ;

Panigrahy, B ;

Ostlund, E ;

Schmitt, B ;

Malkinson, M ;

Banet, C ;

Weissman, J ;

Komar, N ;

Savage, HM ;

Stone, W ;

McNamara, T ;

Gubler, DJ .

SCIENCE, 1999, 286 (5448) :2333-2337

[8]

High-throughput real-time reverse transcription PCR quantitation of hepatitis C virus RNA [J].

Martell, M ;

Gómez, J ;

Esteban, JI ;

Sauleda, S ;

Quer, J ;

Cabot, B ;

Esteban, R ;

Guardia, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (02) :327-332

[9]

Standardization of immunoglobulin M capture enzyme-linked immunosorbent assays for routine diagnosis of arboviral infections [J].

Martin, DA ;

Muth, DA ;

Brown, T ;

Johnson, AJ ;

Karabatsos, N ;

Roehrig, JT .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (05) :1823-1826

[10]

Rapid reverse transcription-PCR detection of hepatitis C virus RNA in serum by using the TaqMan fluorogenic detection system [J].

Morris, T ;

Robertson, B ;

Gallagher, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (12) :2933-2936

← 1 2 →