Quantitation of porcine cytomegalovirus in pig tissues by PCR

被引：35

作者：

Fryer, JFL

Griffiths, PD

Fishman, JA

Emery, VC

Clark, DA

机构：

[1] UCL, Royal Free & Univ Coll Med Sch, Dept Virol, London NW3 2PF, England

[2] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Transplant Infect Dis Program, Boston, MA USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 03期

关键词：

D O I：

10.1128/JCM.39.3.1155-1156.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A quantitative-competitive PCR for the quantification of porcine cytomegalovirus (PCMV) was developed. The virus was detected in a variety of pig organs (including potential xenotransplant donations),,vith viral loads ranging from <10 to: 97 genome copies/<mu>g of DNA. This assay will have significant utility for studying the activation and replication of PCMV and in swine models for allo- and xenotransplantation.

引用

页码：1155 / 1156

页数：2

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