DNA methylation and cellular reprogramming

被引：170

作者：

De Carvalho, Daniel D.

You, Jueng Soo

Jones, Peter A. ^{[1
]}

机构：

[1] Univ So Calif, Keck Sch Med, Norris Comprehens Canc Ctr, Dept Urol, Los Angeles, CA 90033 USA

来源：

TRENDS IN CELL BIOLOGY | 2010年 / 20卷 / 10期

基金：

美国国家卫生研究院;

关键词：

PLURIPOTENT STEM-CELLS; DOMAIN-CONTAINING PROTEINS; HISTONE DEMETHYLATION; DEFINED FACTORS; IPS CELLS; MOUSE; GENERATION; FIBROBLASTS; GENOME; GENES;

D O I：

10.1016/j.tcb.2010.08.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The recent discovery that a small number of defined factors are sufficient to reprogram somatic cells into pluripotent stem cells has significantly expanded our knowledge of the plasticity of the epigenome. In this review we discuss some aspects of cell fate plasticity and epigenetic alterations, with emphasis on DNA methylation during cellular reprogramming. Recent data suggest that DNA methylation is a major barrier to induced pluripotent stem (iPS) cell reprogramming. The demethylating agent 5-azacytidine can enhance the efficiency of iPS cells generation and the putative DNA demethylase protein activation-induced cytidine deaminase (AID/AICDA) can erase DNA methylation at pluripotency gene promoters, thereby allowing cellular reprogramming. Elucidation of the epigenetic changes taking place during cellular reprogramming will enhance our understanding of stem cell biology and facilitate therapeutic applications.

引用

收藏

页码：609 / 617

页数：9

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