Changes in cytokinin distribution in the ovule and ovary of Taraxacum officinale Web. in early stages of embryogenesis

被引:2
作者
Gussakovskaya, MA [1 ]
Blintsov, AN [1 ]
机构
[1] Moscow MV Lomonosov State Univ, Sch Biol, Dept Plant Physiol, Moscow 119899, Russia
基金
俄罗斯基础研究基金会;
关键词
Taraxacum officinale; ovule; ovary; endogenous hormones; zeatin; zeatin riboside; ELISA; immunochemical micro method for differential and quantitative determination of zeatin and zeatin riboside;
D O I
10.1023/A:1010255914540
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Quantitative changes in the hormonal status of the ovules and ovaries were first studied in Taraxacum officinale Web. at the early stages of embryogenesis. The plant material was analyzed by ELISA using labeled anti-rabbit antibodies. A new procedure for differential and quantitative determination of the main endogenous cytokinins based on the estimation of the effective zeatin and zeatin riboside concentrations from calibration curves constructed using zeatin and zeatin riboside as standard antigens was developed. It was shown that, at the three initial stages of embryogenesis examined, the concentration of zeatin uniformly increased in T. officinale ovules. The concentration of zeatin riboside, conversely, uniformly decreased. However, their total concentration changed insignificantly. A gradual increase in the concentration of the active and storage hormone forms from the ovary to the ovule was shown.
引用
收藏
页码:305 / 311
页数:7
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