Lipoteichoic acid and protein-A from Staphylococcus aureus stimulate release of hepatocyte growth factor (HGF) by human dermal fibroblasts

被引:10
作者
Baroni, A
Perfetto, B
Ruocco, E
Rossano, F
机构
[1] Univ Naples, Fac Med & Chirurg 2, Ist Microbiol, I-80137 Napoli, Italy
[2] Univ Naples, Fac Med & Chirurg 2, Ist Clin Dermosifilopat, I-80137 Napoli, Italy
关键词
dermal fibroblasts; HGF; bacterial components;
D O I
10.1007/s004030050292
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
In this study we demonstrated that Staphylococcus aureus lipoteichoic acid (LTA) and protein-A (PA) induce the release from human dermal fibroblasts of hepatocyte growth factor (HGF), a proliferation factor of epithelial cells (including keratinocytes). In contrast, LPS and porins from Pseudomonas aeruginosa did not stimulate HGF production. Recombinant human IL-1 beta induced HGF release. This production was synergistically enhanced when in association with LTA (by more than twice) and PA (by about two-thirds). Controls were performed in the presence of bacterial components alone. In previous studies we have shown that LPS and porins are inducers of IL-1 alpha and beta and other cytokines from human monocytes, Therefore it is possible that in inflammatory cutaneous foci and infected wounds, bacterial components may induce HGF release from dermal human fibroblasts, LTA and PA act directly, while LPS and porins act indirectly, through the release of cytokines by monocytes/macrophages, HGF plays an important role in the repair of cutaneous tissue during gram-positive and gram-negative infections.
引用
收藏
页码:211 / 214
页数:4
相关论文
共 23 条
[1]   POTENT INFLAMMATORY PROPERTIES IN HUMAN SKIN OF INTERLEUKIN-1 ALPHA-LIKE MATERIAL ISOLATED FROM NORMAL SKIN [J].
CAMP, R ;
FINCHAM, N ;
ROSS, J ;
BIRD, C ;
GEARING, A .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1990, 94 (06) :735-741
[2]  
COOPER KD, 1990, J INVEST DERMATOL, V95, P24
[3]   Porins of Pseudomonas aeruginosa induce release of tumor necrosis factor alpha and interleukin-6 by human leukocytes [J].
Cusumano, V ;
Tufano, MA ;
Mancuso, G ;
Carbone, M ;
Rossano, F ;
Fera, MT ;
Ciliberti, FA ;
Ruocco, E ;
Merendino, RA ;
Teti, G .
INFECTION AND IMMUNITY, 1997, 65 (05) :1683-1687
[4]   THE BIOLOGY OF INTERLEUKIN-1 AND COMPARISON TO TUMOR NECROSIS FACTOR [J].
DINARELLO, CA .
IMMUNOLOGY LETTERS, 1987, 16 (3-4) :227-331
[5]   RELEASE OF CYTOKINES INDUCED BY SALMONELLA-TYPHIMURIUM PORINS [J].
GALDIERO, F ;
DELERO, GC ;
BENEDETTO, N ;
GALDIERO, M ;
TUFANO, MA .
INFECTION AND IMMUNITY, 1993, 61 (01) :155-161
[6]  
Galdiero M, 1995, IMMUNOLOGY, V86, P612
[7]   PURIFICATION OF SCATTER FACTOR, A FIBROBLAST-DERIVED BASIC-PROTEIN THAT MODULATES EPITHELIAL INTERACTIONS AND MOVEMENT [J].
GHERARDI, E ;
GRAY, J ;
STOKER, M ;
PERRYMAN, M ;
FURLONG, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (15) :5844-5848
[8]  
GHODA E, 1988, J CLIN INVEST, V81, P414
[9]   IDENTITY OF A TUMOR CYTOTOXIC FACTOR FROM HUMAN FIBROBLASTS AND HEPATOCYTE GROWTH-FACTOR [J].
HIGASHIO, K ;
SHIMA, N ;
GOTO, M ;
ITAGAKI, Y ;
NAGAO, M ;
YASUDA, H ;
MORINAGA, T .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 170 (01) :397-404
[10]  
LUGER TA, 1981, J IMMUNOL, V127, P1493