Evaluation of reference genes for studies of gene expression in human adipose tissue

被引：101

作者：

Gabrielsson, BG

Olofsson, LE

Sjögren, A

Jernås, M

Elander, A

Lönn, M

Rudemo, M

Carlsson, LMS

机构：

[1] Sahlgrens Acad, Res Ctr Endocrinol & Metab, Dept Internal Med, Gothenburg, Sweden

[2] Sahlgrens Acad, Dept Plast Surg, Gothenburg, Sweden

[3] Chalmers Univ Technol, Dept Math Stat, S-41296 Gothenburg, Sweden

来源：

OBESITY RESEARCH | 2005年 / 13卷 / 04期

关键词：

housekeeping; bootstrapping; LRP10; beta-actin; GAPD;

D O I：

10.1038/oby.2005.72

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective: The aim of this study was to evaluate reference genes for expression studies of human adipose tissue. Research Methods and Procedures: Using 52 human adipose tissue expression profiles (HU95), 10 putative reference genes with the lowest variation in expression levels were selected for further studies. Expression stability of these 10 novel and 5 previously established reference genes was evaluated by real-time reverse transcriptase-polymerase chain reaction analysis. For this purpose, 44 adipose tissue biopsies from 27 subjects were chosen to include a wide range of parameters such as sex, age, BMI, depot origin, biopsy procedure, and effects of nutrition. Results: LRP10 was identified as the gene with the least variation in expression levels. The frequently used reference genes RPLPO, 18S rRNA, PPIA, ACTB, and GAPD were ranked as 4, 6, 7, 8, and 10, respectively. Discussion: Our results suggest that LRP10 is a better choice as reference for expression studies of human adipose tissue compared with the most frequently used reference genes.

引用

页码：649 / 652

页数：4

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