Production of C20 polyunsaturated fatty acids (PUFAs) by pathway engineering:: identification of a PUFA elongase component from Caenorhabditis elegans

被引：8

作者：

Beaudoin, F ^{[1
]}

Michaelson, LV ^{[1
]}

Lewis, MJ ^{[1
]}

Shewry, PR ^{[1
]}

Sayanova, O ^{[1
]}

Napier, JA ^{[1
]}

机构：

[1] IACR, Long Ashton Res Stn, Bristol BS41 9AF, Avon, England

来源：

BIOCHEMICAL SOCIETY TRANSACTIONS | 2000年 / 28卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

arachidonic acid; desaturase; fatty acid elongation;

D O I：

10.1042/BST0280661

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using a combination of database-mining and functional characterization, we have identified a component of the polyunsaturated fatty acid (PUFA) elongase. Go-expression of this elongating activitiy with fatty acid desaturases has allowed us to heterologously reconstitute the PUFA biosynthetic pathway. Both these enzymes (desaturases and elongase components) have undergone gene-duplication events which provide a paradigm for the diverged nature of PUFA biosynthetic activities.

引用

页码：661 / 663

页数：3

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