Use of detergents to study membrane rafts: The good, the bad, and the ugly

被引：160

作者：

Shogomori, H ^{[1
]}

Brown, DA ^{[1
]}

机构：

[1] SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA

来源：

BIOLOGICAL CHEMISTRY | 2003年 / 384卷 / 09期

关键词：

detergent insolubility; lipid raft; liquid-ordered; triton X-100; GPI-ANCHORED PROTEINS; CELL-SURFACE; LIPID RAFTS; RESISTANT MEMBRANES; PLASMA-MEMBRANE; DOMAINS; CHOLESTEROL; INSOLUBILITY; ASSOCIATION; TRANSPORT;

D O I：

10.1515/BC.2003.139

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eukaryotic cell membranes contain microdomains called lipid rafts, which are cholesterolrich domains in which lipid acyl chains are tightly packed and highly extended. A variety of proteins associate preferentially with rafts, and this raft association is important in a wide range of functions. A powerful and widely used method for studying lipid rafts takes advantage of their insolubility in nonionic detergents. Here we describe the basis of detergent insolubility, and review strengths, limitations, and unresolved puzzles regarding this method.

引用

页码：1259 / 1263

页数：5

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