Use of detergents to study membrane rafts: The good, the bad, and the ugly

被引:160
作者
Shogomori, H [1 ]
Brown, DA [1 ]
机构
[1] SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA
关键词
detergent insolubility; lipid raft; liquid-ordered; triton X-100; GPI-ANCHORED PROTEINS; CELL-SURFACE; LIPID RAFTS; RESISTANT MEMBRANES; PLASMA-MEMBRANE; DOMAINS; CHOLESTEROL; INSOLUBILITY; ASSOCIATION; TRANSPORT;
D O I
10.1515/BC.2003.139
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Eukaryotic cell membranes contain microdomains called lipid rafts, which are cholesterolrich domains in which lipid acyl chains are tightly packed and highly extended. A variety of proteins associate preferentially with rafts, and this raft association is important in a wide range of functions. A powerful and widely used method for studying lipid rafts takes advantage of their insolubility in nonionic detergents. Here we describe the basis of detergent insolubility, and review strengths, limitations, and unresolved puzzles regarding this method.
引用
收藏
页码:1259 / 1263
页数:5
相关论文
共 30 条
[1]   On the origin of sphingolipid/cholesterol-rich detergent-insoluble cell membranes: Physiological concentrations of cholesterol and sphingolipid induce formation of a detergent-insoluble, liquid-ordered lipid phase in model membranes [J].
Ahmed, SN ;
Brown, DA ;
London, E .
BIOCHEMISTRY, 1997, 36 (36) :10944-10953
[2]   Association of GAP-43 with detergent-resistant membranes requires two palmitoylated cysteine residues [J].
Arni, S ;
Keilbaugh, SA ;
Ostermeyer, AG ;
Brown, DA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (43) :28478-28485
[3]   The immunological synapse [J].
Bromley, SK ;
Burack, WR ;
Johnson, KG ;
Somersalo, K ;
Sims, TN ;
Sumen, C ;
Davis, MM ;
Shaw, AS ;
Allen, PM ;
Dustin, ML .
ANNUAL REVIEW OF IMMUNOLOGY, 2001, 19 :375-396
[4]   SORTING OF GPI-ANCHORED PROTEINS TO GLYCOLIPID-ENRICHED MEMBRANE SUBDOMAINS DURING TRANSPORT TO THE APICAL CELL-SURFACE [J].
BROWN, DA ;
ROSE, JK .
CELL, 1992, 68 (03) :533-544
[5]   TCR signal initiation machinery is pre-assembled and activated in a subset of membrane rafts [J].
Drevot, P ;
Langlet, C ;
Guo, XJ ;
Bernard, AM ;
Colard, O ;
Chauvin, JP ;
Lasserre, R ;
He, HT .
EMBO JOURNAL, 2002, 21 (08) :1899-1908
[6]   The state of lipid rafts: From model membranes to cells [J].
Edidin, M .
ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 2003, 32 :257-283
[7]   Electron spin resonance characterization of liquid ordered phase of detergent-resistant membranes from RBL-2H3 cells [J].
Ge, MT ;
Field, KA ;
Aneja, R ;
Holowka, D ;
Baird, B ;
Freed, JH .
BIOPHYSICAL JOURNAL, 1999, 77 (02) :925-933
[8]   Visualizing lipid raft dynamics and early signaling events during antigen receptor-mediated B-lymphocyte activation [J].
Gupta, N ;
DeFranco, AL .
MOLECULAR BIOLOGY OF THE CELL, 2003, 14 (02) :432-444
[9]   Lipid domain structure of the plasma membrane revealed by patching of membrane components [J].
Harder, T ;
Scheiffele, P ;
Verkade, P ;
Simons, K .
JOURNAL OF CELL BIOLOGY, 1998, 141 (04) :929-942
[10]   Raft membrane domains and immunoreceptor functions [J].
Harder, T .
ADVANCES IN IMMUNOLOGY, VOL 77, 2001, 77 :45-92