Mutational analysis of the PTEN gene in human uterine sarcomas

被引:10
作者
Lancaster, JM
Risinger, JI
Carney, ME
Barrett, JC
Berchuck, A
机构
[1] Duke Univ, Med Ctr, Dept Obstet & Gynecol, Div Gynecol Oncol, Durham, NC 27710 USA
[2] NIEHS, Mol Carcinogenesis Lab, NIH, Res Triangle Pk, NC 27709 USA
关键词
human uterine sarcoma; PTEN gene; SKUT-1B cell line;
D O I
10.1067/mob.2001.114508
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
OBJECTIVE: Uterine sarcomas are rare, lethal cancers, and little is known about their molecular etiology. The PTEN gene is located on chromosome 10q23.3, a region that displays frequent loss of heterozygosity in human uterine sarcomas. PTEN mutations have been described in 40% to 60% of uterine adenocarcinomas. To determine whether the PTEN gene is involved in the pathogenesis of uterine sarcoma, we analyzed deoxyribonucleic acid from uterine sarcomas and cell lines. STUDY DESIGN: Single-strand conformation analysis and direct sequencing of deoxyribonucleic acid were used to screen for PTEN mutations. RESULTS: Silent polymorphisms were detected in 2 of 36 primary uterine sarcomas. A 4-base pair deletion and a point mutation producing a stop codon were identified in 1 cell line. CONCLUSIONS: Mutational inactivation of PTEN does not play a major role in uterine sarcoma tumorigenesis, and another gene or genes on chromosome 10q may be implicated as a cause of these cancers. Differences in the molecular alterations underlying the development of uterine sarcomas and adenocarcinomas are significant.
引用
收藏
页码:1051 / 1053
页数:3
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