Isolation and characterization of an apoptosis-inhibiting component from the hemolymph of Bombyx mori

被引：90

作者：

Kim, EJ ^{[1
]}

Rhee, WJ ^{[1
]}

Park, TH ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Chem Engn, Kwanak Gu, Seoul 151744, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 285卷 / 02期

关键词：

D O I：

10.1006/bbrc.2001.5148

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Silkworm (Bonbyx mori) hemolymph showed an apoptosis-inhibiting activity in insect cells (Sf 9) infected with baculovirus (AcNPV). The addition of silkworm hemolymph into the culture medium increased the host cell longevity due to its apoptosis-inhibition activity. Components with an apoptosis-inhibiting effect were purified from the silkworm hemolymph by heat treatment, gel-filtration chromatography, and ion-exchange chromatography. The component with highest activity was characterized by periodic acid-Schiff staining, isoelectric focusing, MALDI-TOF-mass spectrometry, and N-terminal sequencing and was found to be a nonglycosylated monomeric protein with a molecular weight of ca. 28,000 Da. (C) 2001 Academic Press.

引用

页码：224 / 228

页数：5

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