A high-efficiency protein transduction system demonstrating the role of PKA in long-lasting long-term potentiation

被引:144
作者
Matsushita, M
Tomizawa, K
Moriwaki, A
Li, ST
Terada, H
Matsui, H
机构
[1] Okayama Univ, Sch Med, Dept Physiol 1, Okayama 7008558, Japan
[2] Okayama Univ, Sch Med, Dept Neurol Surg, Okayama 7008558, Japan
关键词
protein transduction; poly arginine; PKA; CREB; LTP; brain slice;
D O I
10.1523/JNEUROSCI.21-16-06000.2001
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Proteins and peptides have been demonstrated to penetrate across the plasma membrane of eukaryotic cells by protein transduction domains. We show that protein transduction by 11 arginine (11R) is an efficient method of delivering proteins into the neurons of brain slices. Here, we demonstrate that PKA inhibitory peptide, fused with 11R and nuclear localization signal, delivers the peptide exclusively into the nuclear compartment of neurons in brain slices. This inhibitory peptide blocked both cAMP responsive element-binding protein phosphorylation and long-lasting long-term potentiation (LTP) induction, but not early LTP. These results highlight transduction of proteins and peptides into specific neuronal subcellular compartments in brain slices as a powerful tool for studying neuronal plasticity.
引用
收藏
页码:6000 / 6007
页数:8
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