Calcitermin, a novel antimicrobial peptide isolated from human airway secretions

被引:70
作者
Cole, AM
Kim, YH
Tahk, S
Hong, T
Weis, P
Waring, AJ
Ganz, T
机构
[1] Univ Calif Los Angeles, Sch Med, Dept Med, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Sch Med, Dept Pediat, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, Sch Med, Will Rogers Inst Pulm Res Lab, Los Angeles, CA 90095 USA
[4] Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Anat Cell Biol & Injury Sci, Newark, NJ 07103 USA
来源
FEBS LETTERS | 2001年 / 504卷 / 1-2期
关键词
antimicrobial peptide; airway; secretion; innate immunity; calgranulin; S100;
D O I
10.1016/S0014-5793(01)02731-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The human airways are protected from pathogenic colonization by a blanket of fluid impregnated with innate antimicrobial effector molecules. Among several previously uncharacterized components, we isolated a peptide that had activity primarily targeting Gram-negative bacteria. We named the peptide 'calcitermin' since its amino acid sequence and mass were equivalent to the 15 C-terminal residues of the S100 protein, calgranulin C. The antimicrobial activity of calcitermin was enhanced in acidic buffers (pH 5.4) and in the presence of micromolar concentrations Of ZnCl2. Analysis revealed a putative zinc-binding consensus sequence as well as an alpha -helical conformation in structure-promoting solvents. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:5 / 10
页数:6
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