Differences in preparative loadability between the charged and uncharged forms of ionizable compounds

被引：47

作者：

Neue, UD ^{[1
]}

Wheat, TE ^{[1
]}

Mazzeo, JR ^{[1
]}

Mazza, CB ^{[1
]}

Cavanaugh, JY ^{[1
]}

Xia, F ^{[1
]}

Diehl, DM ^{[1
]}

机构：

[1] Waters Corp, Milford, MA 01757 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1030卷 / 1-2期

关键词：

preparative chromatography; adsorption isotherms; ionic compounds;

D O I：

10.1016/j.chroma.2003.11.048

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ionizable compounds experience a drastic difference in preparative loadability as a function of pH. It can be shown that the preparative loadability of a compound in the ionic form is by a factor of 20 or more inferior to the loadability of the same compound in the unionized form. In this paper, we demonstrate the reason for this behavior, and show practical applications of the principle. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：123 / 134

页数：12

共 16 条

[1] Overloading study of bases using polymeric RP-HPLC columns as an aid to rationalization of overloading on silica-ODS phases [J].