RNA phage Q beta coat protein as a carrier for foreign epitopes

被引：39

作者：

Kozlovska, TM ^{[1
]}

Cielens, I ^{[1
]}

Vasiljeva, I ^{[1
]}

Strelnikova, A ^{[1
]}

Kazaks, A ^{[1
]}

Dislers, A ^{[1
]}

Dreilina, D ^{[1
]}

Ose, V ^{[1
]}

Gusars, I ^{[1
]}

Pumpens, P ^{[1
]}

机构：

[1] LATVIAN STATE UNIV, BIOMED RES & STUDY CTR, LV-1067 RIGA, LATVIA

来源：

INTERVIROLOGY | 1996年 / 39卷 / 1-2期

关键词：

genetic engineering; cloning; expression; chimeric proteins; RNA phage Q beta; coat proteins;

D O I：

10.1159/000150469

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Q beta gene C has been proposed as a new carrier for the exposure of foreign peptide sequences. Contrary to well-known 'display vectors' on the basis of coat proteins of RNA phage group I, group III phage Q beta-based vectors suggested application of the 195-amino acid extension of coat protein (CP) within the so-called A1 protein for insertion of the appropriate immunological epitopes. 'Mosaic' capsids presenting model hepatitis B virus preS1 and HIV-1 gp120 epitopes and formed by Q beta CP together with A1-derived proteins were obtained as a result of (1) suppression of leaky UGA stop codon of the CP gene and (2) simultaneous expression of 'pure' CP and full-length A1-derived genes obtained after the changing of CP-terminating UGA to strong UAA stop codon or sense GGA codon, respectively.

引用

页码：9 / 15

页数：7

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