Multiplex Real-Time PCR for Detection of an Epidemic KPC-Producing Klebsiella pneumoniae ST258 Clone

被引：43

作者：

Chen, Liang ^{[1
]}

Chavda, Kalyan D. ^{[1
]}

Mediavilla, Jose R. ^{[1
]}

Zhao, Yanan ^{[1
]}

Fraimow, Henry S. ^{[2
]}

Jenkins, Stephen G. ^{[3
]}

Levi, Michael H. ^{[4
,5
]}

Hong, Tao ^{[6
]}

Rojtman, Albert D. ^{[7
]}

Ginocchio, Christine C. ^{[8
]}

Bonomo, Robert A. ^{[9
]}

Kreiswirth, Barry N. ^{[1
]}

机构：

[1] Univ Med & Dent New Jersey, Publ Hlth Res Inst Ctr, Int Ctr Publ Hlth, Newark, NJ 07103 USA

[2] Rowan Univ, Cooper Med Sch, Cooper Univ Hosp, Div Infect Dis, Camden, NJ USA

[3] Weill Cornell Med Coll, Dept Pathol & Lab Med, New York, NY USA

[4] Montefiore Med Ctr, Dept Pathol, Bronx, NY 10467 USA

[5] Albert Einstein Coll Med, Bronx, NY 10467 USA

[6] Hackensack Med Ctr, Dept Pathol, Hackensack, NJ 07604 USA

[7] Jersey Shore Univ, Med Ctr, Dept Pathol, Neptune, NJ USA

[8] N Shore LIJ Hlth Syst Labs, Dept Pathol & Lab Med, Lake Success, NY USA

[9] Louis Stokes Cleveland Vet Affairs Med Ctr, Cleveland, OH USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2012年 / 56卷 / 06期

基金：

美国国家卫生研究院;

关键词：

BLA(KPC) CARBAPENEMASE GENES; RAPID DETECTION; ENTEROBACTERIACEAE; DISSEMINATION; SPREAD;

D O I：

10.1128/AAC.00316-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and bla(KPC) carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and bla(KPC) in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.

引用

页码：3444 / 3447

页数：4

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