Mechanisms underlying the xanthine oxidase inhibitory effects of dietary flavonoids galangin and pinobanksin

被引：77

作者：

Dong, Yi ^{[1
,2
]}

Huang, Huihua ^{[1
]}

Zhao, Mouming ^{[1
,2
,3
]}

Sun-Waterhouse, Dongxiao ^{[1
,2
]}

Lin, Lianzhu ^{[1
,2
]}

Xiao, Chuqiao ^{[1
,2
]}

机构：

[1] S China Univ Technol, Sch Food Sci & Technol, Guangzhou 510640, Guangdong, Peoples R China

[2] Guangdong Food Green Proc & Nutr Regulat Technol, Guangzhou 510640, Guangdong, Peoples R China

[3] S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China

来源：

JOURNAL OF FUNCTIONAL FOODS | 2016年 / 24卷

基金：

中国国家自然科学基金;

关键词：

Galangin; Pinobanksin; Xanthine oxidase inhibition; Fluorescence quenching; Molecular docking; STRUCTURE-AFFINITY RELATIONSHIP; SERUM-ALBUMIN; BINDING; ANTIOXIDANT; EXTRACTS; PROTEINS; RESVERATROL; POLYPHENOLS; KAEMPFEROL; PROPOLIS;

D O I：

10.1016/j.jff.2016.03.021

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

Xanthine oxidase (XOD) inhibitory activities of five dietary flavonoids pinobanksin, galangin, pinocembrin, pinocembrin-7-O-beta-D-glucopyranoside and glabranin were evaluated. Enzyme kinetic studies and molecular docking simulation were conducted to investigate the mechanisms underlying the inhibitory activities. The results showed that these flavonoids exhibited excellent inhibitory activities (which were ranked in the order of pinobanksin > galangin > pinocembrin > pinocembrin-7-O-beta-D-glucopyranoside > glabranin). Competitive inhibition and a mixed-type of competitive-noncompetitive inhibition were observed. The mode of inhibition was dependent on the type and concentration of the substrate and inhibitor. Fluorescence quenching data suggested that these flavonoids could interact with XOD at more than one binding site. The docking simulation revealed that galangin and pinobanksin could enter into the active site of XOD and form hydrogen bonding with amino acid residues (such as Ser-876, Asn-768, Glu-1261 and Thr-1010) and sandwiching aromatic interactions (pi-pi interactions) around the active site of XOD. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：26 / 36

页数：11

共 43 条

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