Immune response of goats immunised with glutathione S-transferase and experimentally challenged with Fasciola hepatica

被引：18

作者：

Buffoni, L. ^{[1
]}

Zafra, R. ^{[2
]}

Perez-Ecija, A. ^{[2
]}

Martinez-Moreno, F. J. ^{[1
]}

Martinez-Galisteo, E. ^{[3
]}

Moreno, T. ^{[1
]}

Perez, J. ^{[2
]}

Martinez-Moreno, A. ^{[1
]}

机构：

[1] Univ Cordoba, Anim Hlth Dept Parasitol, Fac Vet Med, Cordoba 14014, Spain

[2] Univ Cordoba, Anat & Comparat Pathol Dept, Fac Vet Med, Cordoba 14014, Spain

[3] Univ Cordoba, Dept Biochem & Mol Biol, Fac Vet Med, Cordoba 14014, Spain

来源：

PARASITOLOGY INTERNATIONAL | 2010年 / 59卷 / 02期

关键词：

Fasciola hepatica; Immunisation; FhGST; Goats; AVIUM SUBSP PARATUBERCULOSIS; CATHEPSIN-L PROTEINASES; EXPERIMENTAL-INFECTION; IFN-GAMMA; TGF-BETA; VACCINATION; CATTLE; GIGANTICA; PROTECTION; ANTIGEN;

D O I：

10.1016/j.parint.2009.12.005

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程]; 100103 [病原生物学];

摘要：

Glutathione 5-transferase (FhGST) purified from Fasciola hepatica adult worms was used to immunise goats against F. hepatica in an experimental infection: the level of protection, in terms of fluke burden, faecal egg counts and hepatic damage was determined, as well as the humoral and cellular immune response elicited. Animals were allocated into three groups of six animals each: group 1 (immunised with FhGST and infected), group 2 (unimmunised and infected), and group 3 (unimmunised and uninfected). There was no significant reduction of fluke burden (9.3%) or faecal egg counts; hepatic damage was also similar in both infected groups. However, immunisation with FhGST induced the development of a well-defined immune response, characterized by the production of specific-FhGST antibodies as well as the appearance of circulating IL-4. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：147 / 153

页数：7

共 37 条

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Vaccination against Fasciola hepatica infection using a Schistosoma mansoni defined recombinant antigen, Sm14 [J].