Molecular analysis of bovine spongiform encephalopathy and scrapie strain variation

被引：67

作者：

Kuczius, T ^{[1
]}

Haist, I ^{[1
]}

Groschup, MH ^{[1
]}

机构：

[1] Fed Res Ctr Virus Dis Anim, D-72001 Tubingen, Germany

来源：

JOURNAL OF INFECTIOUS DISEASES | 1998年 / 178卷 / 03期

关键词：

D O I：

10.1086/515337

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Five mouse scrapie strains, a mouse-passaged scrapie isolate derived from a field case in sheep in Germany, and 2 mouse-passaged bovine spongiform encephalopathy (BSE) isolates were analyzed by immunoblot in regards to banding patterns of proteinase K-digested pathologic prion proteins (PrPres). To obtain reliable results, the photo-imager technique was used for measurement of staining band intensities. Distinct and reproducible profiles were observed for the different strains or isolates. A British and a German BSE isolate were similar, suggesting the same source of infection. The German scrapie isolate resembled scrapie strain ME7, which has frequently been isolated from sheep scrapie in the past. In selected strains or isolates, no influence of the mouse lines used was observed on PrPres profiles, nor were brain region-specific differences apparent. This investigation suggests that PrPres glycotyping can be an invaluable tool for the in vitro differentiation of BSE and scrapie isolates.

引用

收藏

页码：693 / 699

页数：7

相关论文

共 35 条

[1] SCRAPIE AND CELLULAR PRP ISOFORMS ARE ENCODED BY THE SAME CHROMOSOMAL GENE [J].

BASLER, K ;

OESCH, B ;

SCOTT, M ;

WESTAWAY, D ;

WALCHLI, M ;

GROTH, DF ;

MCKINLEY, MP ;

PRUSINER, SB ;

WEISSMANN, C .

CELL, 1986, 46 (03) :417-428

[2] DISTINCT PRP PROPERTIES SUGGEST THE MOLECULAR-BASIS OF STRAIN VARIATION IN TRANSMISSIBLE MINK ENCEPHALOPATHY [J].

BESSEN, RA ;

MARSH, RF .

JOURNAL OF VIROLOGY, 1994, 68 (12) :7859-7868

[3] IMMUNOLOGICAL ANALYSIS OF HOST AND AGENT EFFECTS ON CREUTZFELDT-JAKOB DISEASE AND SCRAPIE PRION PROTEINS [J].

BOCKMAN, JM ;

KINGSBURY, DT .

JOURNAL OF VIROLOGY, 1988, 62 (09) :3120-3127

[4] TRANSMISSION OF BOVINE SPONGIFORM ENCEPHALOPATHY AND SCRAPIE TO MICE - STRAIN VARIATION AND THE SPECIES BARRIER [J].

BRUCE, M ;

CHREE, A ;

MCCONNELL, I ;

FOSTER, J ;

PEARSON, G ;

FRASER, H .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1994, 343 (1306) :405-411

[5] CEREBRAL AMYLOIDOSIS IN SCRAPIE IN MOUSE - EFFECT OF AGENT STRAIN AND MOUSE GENOTYPE [J].

BRUCE, ME ;

DICKINSON, AG ;

FRASER, H .

NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, 1976, 2 (06) :471-478

[6] THE DISEASE CHARACTERISTICS OF DIFFERENT STRAINS OF SCRAPIE IN SINC CONGENIC MOUSE LINES - IMPLICATIONS FOR THE NATURE OF THE AGENT AND HOST CONTROL OF PATHOGENESIS [J].

BRUCE, ME ;

MCCONNELL, I ;

FRASER, H ;

DICKINSON, AG .

JOURNAL OF GENERAL VIROLOGY, 1991, 72 :595-603

[7]

BRUCE ME, 1987, J GEN VIROL, V68, P78

[8]

Bruce ME, 1979, SLOW TRANSMISSIBLE D, V2, P71

[9] PRION ISOLATE SPECIFIED ALLOTYPIC INTERACTIONS BETWEEN THE CELLULAR AND SCRAPIE PRION PROTEINS IN CONGENIC AND TRANSGENIC MICE [J].

CARLSON, GA ;

EBELING, C ;

YANG, SL ;

TELLING, G ;

TORCHIA, M ;

GROTH, D ;

WESTAWAY, D ;

DEARMOND, SJ ;

PRUSINER, SB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (12) :5690-5694

[10] GENETICS AND POLYMORPHISM OF THE MOUSE PRION GENE-COMPLEX - CONTROL OF SCRAPIE INCUBATION-TIME [J].

CARLSON, GA ;

GOODMAN, PA ;

LOVETT, M ;

TAYLOR, BA ;

MARSHALL, ST ;

PETERSONTORCHIA, M ;

WESTAWAY, D ;

PRUSINER, SB .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (12) :5528-5540

← 1 2 3 4 →