Recognition of CD52 allelic gene products by CAMPATH-1H antibodies

被引：29

作者：

Hale, C

Bartholomew, M

Taylor, V

Stables, J

Topley, P

Tite, J

机构：

[1] WELLCOME RES LABS,MOLEC IMMUNOL GRP,BECKENHAM BR3 3BS,KENT,ENGLAND

[2] WELLCOME RES LABS,EXPT BIOL GRP,DIV BIOL,BECKENHAM BR3 3BS,KENT,ENGLAND

来源：

IMMUNOLOGY | 1996年 / 88卷 / 02期

关键词：

D O I：

10.1111/j.1365-2567.1996.tb00003.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Cloning of the CD52 from a B-lymphocyte tumour cDNA library revealed two closely related sequences differing only at two amino acids C-terminal to the proposed point of glycosylphosphatidylinositol (GPI)-linkage. When transfected into CHO cells only one of these sequences gave high-level expression of the antigen recognized by the prototypic anti-CD52 antibody CAMPATH-1 whereas in JURKAT cells good expression levels were obtained with both sequences, Fusion of the sequence from the second sequence to DNA encoding the extracellular domain of CD4 indicated that this sequence was capable of directing GPI linkage. The possible implications for the function of CD52 and serotherapy with anti-CD52 antibodies are discussed.

引用

页码：183 / 190

页数：8

共 13 条

[1] CD59, AN LY-6-LIKE PROTEIN EXPRESSED IN HUMAN LYMPHOID-CELLS, REGULATES THE ACTION OF THE COMPLEMENT MEMBRANE ATTACK COMPLEX ON HOMOLOGOUS CELLS [J].