Improvement of blood and fly gut processing for PCR diagnosis of trypanosomosis

被引：31

作者：

Penchenier, L

Dumas, V

Grebaut, P

Reifenberg, JM

Cuny, G

机构：

[1] OCEAC, LAB RECH TRYPANOSOMES, YAOUNDE, CAMEROON

[2] ORSTOM, RETROVIRUS LAB, F-34032 MONTPELLIER 1, FRANCE

[3] ORSTOM, CIRAD, EMVT, LAB PTHOL TROP, F-34032 MONTPELLIER 1, FRANCE

[4] ORSTOM, LAB EPIDEMIOL MALAD VECTEURS, F-34032 MONTPELLIER 1, FRANCE

来源：

PARASITE | 1996年 / 3卷 / 04期

关键词：

PCR; diagnosis; Trypanosoma;

D O I：

10.1051/parasite/1996034387

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学]; 100103 [病原生物学];

摘要：

We have adapted a simple and efficient technique to detect trypanosomes in human blood, without DNA purification, and increased the sensitivity threshold to 1 parasite in 1 ml. We have then applied it for detection of parasites in midguts of tsetse flies, negative by microscopy. This technique has been developed for field conditions and could greatly facilitate epidemiological studies.

引用

页码：387 / 389

页数：3

共 9 条

[1]

SPECIES-SPECIFIC DNA PROBES FOR THE IDENTIFICATION OF AFRICAN TRYPANOSOMES IN TSETSE FLIES [J].