X-ray structure of a tetranucleosome and its implications for the chromatin fibre

被引:588
作者
Schalch, T [1 ]
Duda, S [1 ]
Sargent, DF [1 ]
Richmond, TJ [1 ]
机构
[1] ETH Honggerberg, ETH Zurich, Inst Mol Biol & Biophys, CH-8093 Zurich, Switzerland
关键词
D O I
10.1038/nature03686
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
DNA in eukaryotic chromosomes is organized in arrays of nucleosomes compacted into chromatin fibres. This higher-order structure of nucleosomes is the substrate for DNA replication, recombination, transcription and repair. Although the structure of the nucleosome core is known at near-atomic resolution(1), even the most fundamental information about the organization of nucleosomes in the fibre is controversial. Here we report the crystal structure of an oligonucleosome (a compact tetranucleosome) at 9 angstrom resolution, solved by molecular replacement using the nucleosome core structure. The structure shows that linker DNA zigzags back and forth between two stacks of nucleosome cores, which forma truncated two-start helix, and does not follow a path compatible with a one-start solenoidal helix(2). The length of linker DNA is most probably buffered by stretching of the DNA contained in the nucleosome cores. We have built continuous fibre models by successively stacking tetranucleosomes one on another. The resulting models are nearly fully compacted and most closely resemble the previously described crossed-linker model(3). They suggest that the interfaces between nucleosomes along a single helix start are polymorphic.
引用
收藏
页码:138 / 141
页数:4
相关论文
共 30 条
[1]   HIGHER-ORDER STRUCTURE IN A SHORT REPEAT LENGTH CHROMATIN [J].
ALLAN, J ;
RAU, DC ;
HARBORNE, N ;
GOULD, H .
JOURNAL OF CELL BIOLOGY, 1984, 98 (04) :1320-1327
[2]   THE DIAMETERS OF FROZEN-HYDRATED CHROMATIN FIBERS INCREASE WITH DNA LINKER LENGTH - EVIDENCE IN SUPPORT OF VARIABLE DIAMETER MODELS FOR CHROMATIN [J].
ATHEY, BD ;
SMITH, MF ;
RANKERT, DA ;
WILLIAMS, SP ;
LANGMORE, JP .
JOURNAL OF CELL BIOLOGY, 1990, 111 (03) :795-806
[3]   ELECTROSTATIC MECHANISM OF NUCLEOSOME SPACING [J].
BLANK, TA ;
BECKER, PB .
JOURNAL OF MOLECULAR BIOLOGY, 1995, 252 (03) :305-313
[4]  
Brunger AT, 1998, ACTA CRYSTALLOGR D, V54, P905, DOI 10.1107/s0907444998003254
[5]   CHANGES IN CHROMATIN FOLDING IN SOLUTION [J].
BUTLER, PJG ;
THOMAS, JO .
JOURNAL OF MOLECULAR BIOLOGY, 1980, 140 (04) :505-529
[6]   Solvent mediated interactions in the structure of the nucleosome core particle at 1.9 Å resolution [J].
Davey, CA ;
Sargent, DF ;
Luger, K ;
Maeder, AW ;
Richmond, TJ .
JOURNAL OF MOLECULAR BIOLOGY, 2002, 319 (05) :1097-1113
[7]   Nucleosome arrays reveal the two-start organization of the chromatin fiber [J].
Dorigo, B ;
Schalch, T ;
Kulangara, A ;
Duda, S ;
Schroeder, RR ;
Richmond, TJ .
SCIENCE, 2004, 306 (5701) :1571-1573
[8]   Chromatin fiber folding: Requirement for the histone H4N-terminal tail [J].
Dorigo, B ;
Schalch, T ;
Bystricky, K ;
Richmond, TJ .
JOURNAL OF MOLECULAR BIOLOGY, 2003, 327 (01) :85-96
[9]   SOLENOIDAL MODEL FOR SUPERSTRUCTURE IN CHROMATIN [J].
FINCH, JT ;
KLUG, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (06) :1897-1901
[10]   CHROMATIN HIGHER-ORDER STRUCTURE STUDIED BY NEUTRON-SCATTERING AND SCANNING-TRANSMISSION ELECTRON-MICROSCOPY [J].
GERCHMAN, SE ;
RAMAKRISHNAN, V .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (22) :7802-7806