An acid dissociation bridging ELISA for detection of antibodies directed against therapeutic proteins in the presence of antigen

被引：157

作者：

Patton, A ^{[1
]}

Mullenix, MC ^{[1
]}

Swanson, SJ ^{[1
]}

Koren, E ^{[1
]}

机构：

[1] Amgen Inc, Dept Clin Immunol, Thousand Oaks, CA 91320 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2005年 / 304卷 / 1-2期

关键词：

antibody assay; acid dissociation; therapeutic proteins; ELISA; antigen interference;

D O I：

10.1016/j.jim.2005.06.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bridging Enzyme-Linked Immunosorbent Assay (ELISA) is commonly used in detection of antibodies directed against therapeutic proteins. Advantages of the bridging ELISA include the capability to detect antibodies regardless of their isotype or the species of origin. However, detection of antibodies can be difficult, if not impossible, in the presence of high levels of the antigen in the sample matrix. This protocol describes a bridging ELISA that uses a covalently coupled high density antigen surface combined with an acid dissociation step to allow for antibody detection in the presence of antigen in human serum. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：189 / 195

页数：7

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