Identification of a novel class of genomic DNA-binding sites suggests a mechanism for selectivity in target gene activation by the tumor suppressor protein p53

被引:102
作者
Resnick-Silverman, L
St Clair, S
Maurer, M
Zhao, K
Manfredi, JJ [1 ]
机构
[1] CUNY Mt Sinai Sch Med, Derald H Ruttenberg Canc Ctr, New York, NY 10029 USA
[2] CUNY Mt Sinai Sch Med, Brookdale Ctr Mol & Dev Biol, New York, NY 10029 USA
关键词
tumor suppressor; DNA binding; sequence specificity; p53; protein; transcriptional activation; binding sites;
D O I
10.1101/gad.12.14.2102
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
There are two response elements for p53 in the promoter of the gene for the cyclin-dependent kinase inhibitor p21. The binding of p53 to the 5' site was enhanced by incubation with monoclonal antibody 421, whereas the binding of p53 to the 3' site was inhibited. Mutational analysis showed that a single-base change caused one element to behave like the other. ih response element in the human cdc25C promoter is bound by p53 with properties similar to the 3' site. These results identify two classes of p53-binding sites and suggest a mechanism for target gene selectivity by p53.
引用
收藏
页码:2102 / 2107
页数:6
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