Antibody recognition of specific crystal faces of 1,4-dinitrobenzene

被引:15
作者
Bromberg, R [1 ]
Kessler, N [1 ]
Addadi, L [1 ]
机构
[1] Weizmann Inst Sci, Dept Biol Struct, IL-76100 Rehovot, Israel
关键词
immunogenicity; crystal; dinitrobenzene; monoclonal antibodies; sublimation; molecular recognition;
D O I
10.1016/S0022-0248(98)00517-X
中图分类号
O7 [晶体学];
学科分类号
0702 ; 070205 ; 0703 ; 080501 ;
摘要
Two independent techniques have been developed and applied to determine the crystal face specificity, if any, of different monoclonal antibodies that were raised and selected based on their affinity to crystal surfaces. The techniques used are: (a) in situ detection of color development by ELISA, following antibody adsorption on mature crystals; (b) morphological modifications induced by crystal growth in the presence of antibody. In addition, surface sublimation patterns were observed, induced by antibody adsorbed during crystal growth. One antibody, specific to crystals of 1,4-dinitrobenzene (DNB), is shown by all techniques to selectively recognize one face of the crystals. A nonspecific antibody and an antibody specific to cholesterol (but not to DNB) crystals, have different effects and display no crystal face specificity. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:656 / 664
页数:9
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