Effects of brain ischemia on intermediate filaments of rat hippocampus

被引:10
作者
CamargoDeMorais, M [1 ]
DeFreitas, M [1 ]
DeMattos, AG [1 ]
Schroder, N [1 ]
Zilles, AC [1 ]
Lisboa, CSF [1 ]
Arteni, N [1 ]
Barlem, A [1 ]
Schierholt, R [1 ]
Zwetsch, G [1 ]
Souza, CAB [1 ]
PessoaPureur, R [1 ]
Netto, CA [1 ]
机构
[1] UNIV FED RIO GRANDE SUL,IB,DEPT BIOQUIM,BR-90046900 PORTO ALEGRE,RS,BRAZIL
关键词
neurofilaments; GFAP; brain ischemia; hippocampus; cytoskeleton; neuronal death;
D O I
10.1007/BF02527758
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Neurofilaments subunits (NF-H, NF-M, NF-L) and glial fibrillary acidic protein (GFAP) were investigated in the hippocampus of rats after distinct periods of reperfusion (1 to 15 days) following 20 min of transient global forebrain ischemia in the rat. In vitro [Ca-14]leucine incorporation was not altered until 48 h after the ischemic insult, however concentration of intermediate filament subunits significantly decreased in this period. Three days after the insult, leucine incorporation significantly increased while the concentration NF-H, NF-M, and NF-L were still diminished after 15 days of reperfusion. In vitro incorporation of P-32 into NF-M and NF-L suffered immediately after ischemia, but returned to control values after two days of reperfusion. GFAP levels decreased immediately after ischemia but quickly recovered and significantly peaked from 7 to 10 days after the insult. These results suggest that transient ischemia followed by reperfusion causes proteolysis of intermediate filaments in the hippocampus, and that proteolysis could be facilitated by diminished phosphorylation levels of NF-M and NF-L.
引用
收藏
页码:595 / 602
页数:8
相关论文
共 49 条
  • [1] ASSEMBLY AND EXCHANGE OF INTERMEDIATE FILAMENT PROTEINS OF NEURONS - NEUROFILAMENTS ARE DYNAMIC STRUCTURES
    ANGELIDES, KJ
    SMITH, KE
    TAKEDA, M
    [J]. JOURNAL OF CELL BIOLOGY, 1989, 108 (04) : 1495 - 1506
  • [2] A BRIEF PERIOD OF HYPOXIA CAUSES PROTEOLYSIS OF CYTOSKELETAL PROTEINS IN HIPPOCAMPAL SLICES
    ARAI, A
    VANDERKLISH, P
    KESSLER, M
    LEE, K
    LYNCH, G
    [J]. BRAIN RESEARCH, 1991, 555 (02) : 276 - 280
  • [3] EFFECT OF PROTEIN-MALNUTRITION ON GLYCOPROTEIN, PROTEIN AND LIPID-SYNTHESIS IN THE RAT CEREBELLUM DURING THE PERIOD OF BRAIN GROWTH SPURT
    AZZOLIN, IR
    BERNARD, EA
    TRINDADE, VMT
    GAMALLO, JLG
    PERRY, MLS
    [J]. ANNALS OF NUTRITION AND METABOLISM, 1991, 35 (02) : 82 - 88
  • [4] CALPAIN AS A NOVEL TARGET FOR TREATING ACUTE NEURODEGENERATIVE DISORDERS
    BARTUS, RT
    ELLIOTT, PJ
    HAYWARD, NJ
    DEAN, RL
    HARBESON, S
    STRAUB, JA
    LI, Z
    POWERS, JC
    [J]. NEUROLOGICAL RESEARCH, 1995, 17 (04) : 249 - 258
  • [5] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [6] BRADY ST, 1992, NEUROREGENERATION, P7
  • [7] CHOI DW, 1990, J NEUROSCI, V10, P2493
  • [8] CHOI DW, 1990, ANNU REV NEUROSCI, V13, P171, DOI 10.1146/annurev.neuro.13.1.171
  • [9] CYTOSKELETAL-ASSOCIATED PROTEIN-KINASE AND PHOSPHATASE-ACTIVITIES FROM CEREBRAL-CORTEX OF YOUNG-RATS
    DEFREITAS, MS
    DEMATTOS, AG
    CAMARGO, MM
    WANNMACHER, CMD
    PESSOAPUREUR, R
    [J]. NEUROCHEMICAL RESEARCH, 1995, 20 (08) : 951 - 956
  • [10] PHOSPHORYLATION MODULATES CALPAIN-MEDIATED PROTEOLYSIS AND CALMODULIN-BINDING OF THE 200-KDA AND 160-KDA NEUROFILAMENT PROTEINS
    GREENWOOD, JA
    TRONCOSO, JC
    COSTELLO, AC
    JOHNSON, GVW
    [J]. JOURNAL OF NEUROCHEMISTRY, 1993, 61 (01) : 191 - 199