Direct observation of affinity reactions by reflected-mode operation of integrated optical grating coupler

被引：35

作者：

Brandenburg, A ^{[1
]}

Polzius, R ^{[1
]}

Bier, F ^{[1
]}

Bilitewski, U ^{[1
]}

Wagner, E ^{[1
]}

机构：

[1] GESELL BIOTECHNOL FORSCH MBH,W-3300 BRAUNSCHWEIG,GERMANY

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1996年 / 30卷 / 01期

关键词：

affinity reactions; grating couplers; integrated optics; reflected-mode operation;

D O I：

10.1016/0925-4005(95)01747-J

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The reflected mode of operation of grating coupler measurement is demonstrated to be feasible for real-time monitoring of biochemical reactions, especially reactions of protein G and human IgG. The noise level of the system is delta n(eff) = 3 x 10(-6). The detection limit is calculated as 0.2 mu g ml(-1) or 1.4 x 10(-9) M human IgG. Due to the ease of handling and the simple set-up with relatively cheap components, it should be possible to provide a cost-efficient analytical tool.

引用

页码：55 / 59

页数：5

共 10 条

[1]

BIER FF, IN PRESS ANALYST

[2]

BJORCK L, 1984, J IMMUNOL, V133, P969

[3] GRATING COUPLERS AS CHEMICAL SENSORS - A NEW OPTICAL CONFIGURATION [J].

BRANDENBURG, A ;

GOMBERT, A .

SENSORS AND ACTUATORS B-CHEMICAL, 1993, 17 (01) :35-40

[4] SPECIFIC BINDING OF PHOTOSYNTHETIC REACTION CENTERS TO HERBICIDE-MODIFIED GRATING COUPLERS [J].

JOCKERS, R ;

BIER, FF ;

SCHMID, RD .

ANALYTICA CHIMICA ACTA, 1993, 280 (01) :53-59

[5] OUTPUT GRATING COUPLERS ON PLANAR OPTICAL WAVE-GUIDES AS DIRECT IMMUNOSENSORS [J].

LUKOSZ, W ;

CLERC, D ;

NELLEN, PM ;

STAMM, C ;

WEISS, P .

BIOSENSORS & BIOELECTRONICS, 1991, 6 (03) :227-232

[6] OUTPUT GRATING COUPLERS ON PLANAR WAVE-GUIDES AS INTEGRATED OPTICAL CHEMICAL SENSORS [J].

LUKOSZ, W ;

NELLEN, PM ;

STAMM, C ;

WEISS, P .

SENSORS AND ACTUATORS B-CHEMICAL, 1990, 1 (1-6) :585-588

[7]

Lukosz W., 1983, IEE C PUBL LONDON, V227, P152

[8] INTEGRATED OPTICAL INPUT GRATING COUPLERS AS BIOCHEMICAL SENSORS [J].

NELLEN, PM ;

TIEFENTHALER, K ;

LUKOSZ, W .

SENSORS AND ACTUATORS, 1988, 15 (03) :285-295

[9] ONLINE MONITORING OF MONOCLONAL-ANTIBODIES IN ANIMAL-CELL CULTURE USING A GRATING COUPLER [J].

POLZIUS, R ;

BIER, FF ;

BILITEWSKI, U ;

JAGER, V ;

SCHMID, RD .

BIOTECHNOLOGY AND BIOENGINEERING, 1993, 42 (11) :1287-1292

[10] SENSITIVITY OF GRATING COUPLERS AS INTEGRATED-OPTICAL CHEMICAL SENSORS [J].

TIEFENTHALER, K ;

LUKOSZ, W .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS, 1989, 6 (02) :209-220

← 1 →