Oil extraction from microalgae for biodiesel production

This study examines the performance of supercritical carbon dioxide (SCCO2) extraction and hexane extraction of lipids from marine Chlorococcum sp. for lab-scale biodiesel production. Even though the strain of Chlorococcum sp. used in this study had a low maximum lipid yield (7.1 wt% to dry biomass), the extracted lipid displayed a suitable fatty acid profile for biodiesel [C18:1 (similar to 63 wt%), C16:0 (similar to 19 wt%), C18:2 (similar to 4 wt%), C16:1 (similar to 4 wt%), and C18:0 (similar to 3 wt%)]. For SCCO2 extraction, decreasing temperature and increasing pressure resulted in increased lipid yields. The mass transfer coefficient (k) for lipid extraction under supercritical conditions was found to increase with fluid dielectric constant as well as fluid density. For hexane extraction, continuous operation with a Soxhlet apparatus and inclusion of isopropanol as a co-solvent enhanced lipid yields. Hexane extraction from either dried microalgal powder or wet microalgal paste obtained comparable lipid yields. (C) 2010 Elsevier Ltd. All rights reserved.