A possible role of glutathione and glutathione disulfide in tracheary element differentiation in the cultured mesophyll cells of Zinnia elegans

被引：31

作者：

Henmi, K

Tsuboi, S

Demura, T

Fukuda, H

Iwabuchi, M

Ogawa, K

机构：

[1] RIBS, Okayama 7161241, Japan

[2] Okayama Univ, Fac Pharmaceut Sci, Okayama 7008530, Japan

[3] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo 1130033, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2001年 / 42卷 / 06期

基金：

日本学术振兴会;

关键词：

L-buthionine sulfoximine; glutathione; gamma-glutamylcysteine syntethase (EC 6.3.2.2); redox; tracheary element differentiation; Zinnia elegans;

D O I：

10.1093/pcp/pce072

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We investigated the relationship between the cellular redox state of GSH or GSSG and tracheary element (TE) differentiation using a Zinnia experimental system, in which isolated mesophyll cells transdifferentiate to TEs. TE differentiation was suppressed by the application of L-buthionine sulfoximine (BSO), a potent inhibitor of GSH biosynthesis, at the early stage of cell culture. Application of GSSG at the early culture stage promoted the differentiation, but that of GSH or GSSG at an advanced period of culture suppressed the differentiation. Application of GSH and GSSG nullified the TE differentiation-suppressing effect of BSO, The results suggest that changes in the redox states of GSH and GSSG have a role in TE differentiation.

引用

页码：673 / 676

页数：4

共 22 条

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