Sequences of versatile broad-host-range vectors of the RK2 family

被引：26

作者：

Scott, HN ^{[1
]}

Laible, PD ^{[1
]}

Hanson, DK ^{[1
]}

机构：

[1] Argonne Natl Lab, Biosci Div, Argonne, IL 60439 USA

来源：

PLASMID | 2003年 / 50卷 / 01期

关键词：

mobilizable vector; RK2; pRK404; plasmid DNA sequence; cloning vector; conjugation;

D O I：

10.1016/S0147-619X(03)00030-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Plasmid pRK404-a smaller derivative of RK2-is a tetracycline-resistant broad-host-range vector that carries a multiple cloning site and the lacZ(alpha) peptide that enables blue/white selection for cloned inserts in Escherichia coli. We present herein the complete and annotated sequence of pRK404 and three related vectors-pRK437, pRK442, and pRK442(H). These derivatives have proven to be valuable tools for genetic manipulation in Gram-negative bacteria. The knowledge of their complete sequences will facilitate efficient future engineering of them and will enhance their general applicability to the design of genetic systems for use in organisms for which new genomic sequence data are becoming available. (C) 2003 Elsevier Science (USA). All rights reserved.

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页码：74 / 79

页数：6

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