Cloning and expression analyses of AtMRP4 a novel MRP-like gene from Arabidopsis thaliana

被引：32

作者：

Sánchez-Fernández, R

Ardiles-Díaz, W

Van Montagu, M

Inzé, D

May, MJ

机构：

[1] State Univ Ghent VIB, Dept Genet, Genet Lab, B-9000 Ghent, Belgium

[2] Univ Ghent, Inst Natl Rech Agron France, Lab Associe, B-9000 Ghent, Belgium

来源：

MOLECULAR AND GENERAL GENETICS | 1998年 / 258卷 / 06期

关键词：

Arabidopsis thaliana; glutathione-conjugate pump; stress; xenobiotic detoxification;

D O I：

10.1007/s004380050779

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In all organisms glutathione-conjugate transporters (GS-X pumps) mediate the detoxification of a number of xenobiotics by removing them from the cytosol. In addition, GS-X pumps appear to play a role in the processing of endogenous compounds. We have isolated a novel genomic clone from Arabidopsis thaliana that encodes a putative GS-X pump, AtMRP4, which is part of a recently defined gene family. The derived amino acid sequence shares high levels of similarity (55-63%) with human, yeast, and other Arabidopsis homologues. The expression of the different members of the AtMRP gene family in Arabidopsis cell suspensions after treatment with chemicals that modify glutathione metabolism (compounds that induce different types of stress and that act as herbicide antidotes safeners - in monocotyledonous species) revealed that the members of this gene family are differentially regulated.

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页码：655 / 662

页数：8

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